Detailed information of TA system TA022969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5194631..5195226
• Replicon: chromosome
• Accession: NC_011004
• Organism: Rhodopseudomonas palustris TIE-1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B3Q7W1
• Locus tag: RPAL_RS23875
• Protein ID: WP_012497552.1
• Coordinates: 5194631..5194909 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RPAL_RS23880
• Protein ID: WP_012497553.1
• Coordinates: 5194921..5195226 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RPAL_RS23860	5190666..5193701	+	3036	WP_012497550.1	excinuclease ABC subunit UvrB	-
RPAL_RS23865	5193792..5194025	+	234	WP_011159878.1	DUF1902 domain-containing protein	-
RPAL_RS23870	5194026..5194220	+	195	WP_012497551.1	type II toxin-antitoxin system HicA family toxin	-
RPAL_RS23875	5194631..5194909	+	279	WP_012497552.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RPAL_RS23880	5194921..5195226	+	306	WP_012497553.1	HigA family addiction module antidote protein	Antitoxin
RPAL_RS23885	5195340..5195693	-	354	WP_150104575.1	(2Fe-2S) ferredoxin domain-containing protein	-
RPAL_RS23890	5196082..5197335	-	1254	WP_012497555.1	acetyl-CoA C-acetyltransferase	-
RPAL_RS23895	5197549..5197983	+	435	WP_012497556.1	hypothetical protein	-
RPAL_RS23900	5198467..5198898	+	432	WP_012497557.1	nuclear transport factor 2 family protein	-
RPAL_RS23905	5199106..5199546	+	441	WP_012497558.1	hypothetical protein	-
RPAL_RS27885	5199696..5199842	-	147	WP_011159884.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10913.43 Da
• Isoelectric Point: 9.0805

>T22969 WP_012497552.1 NC_011004:5194631-5194909 [Rhodopseudomonas palustris TIE-1]
MIRSFRNPATARLFADEDVPRFRAIERQARRKLLLLDGAAKLDDLRQPPGNRLEALKGDRRGQYSIRINDQWRICFEWRE
DGAEMVEIVDYH

Nucleotide

• Length: 279 bp

>T22969 NC_011004:5194631-5194909 [Rhodopseudomonas palustris TIE-1]
ATGATCCGCTCGTTCCGAAATCCGGCGACCGCGCGGCTGTTTGCTGATGAGGACGTGCCGCGGTTTCGCGCGATCGAGCG
GCAGGCTCGTCGAAAGCTGCTGCTCCTGGACGGCGCCGCCAAACTCGACGATTTGCGGCAACCTCCCGGCAACAGGCTCG
AGGCTTTGAAGGGCGATCGCCGCGGCCAATACTCGATCCGCATCAACGATCAATGGCGGATTTGCTTCGAGTGGCGTGAG
GACGGCGCCGAAATGGTTGAAATCGTGGACTATCATTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11312.14 Da
• Isoelectric Point: 10.2943

>AT22969 WP_012497553.1 NC_011004:5194921-5195226 [Rhodopseudomonas palustris TIE-1]
MAKPKQLPPIPPGEILIEEFMRPNGISQNRLARDIDINPARVNDIVHGRSAITAAVALRLAKYFGTTPELWMNLQASYDL
RRARAADWPAIEPRVRVLAAE

Nucleotide

• Length: 306 bp

>AT22969 NC_011004:5194921-5195226 [Rhodopseudomonas palustris TIE-1]
ATGGCCAAACCAAAGCAGCTCCCCCCGATCCCTCCCGGCGAGATTCTGATCGAGGAATTCATGCGCCCGAACGGGATCAG
TCAGAACCGGCTGGCACGCGATATCGACATCAACCCGGCTCGGGTCAACGACATCGTGCACGGCCGCTCTGCGATCACCG
CCGCAGTCGCCCTGCGGCTGGCGAAGTACTTCGGCACGACGCCCGAACTCTGGATGAACTTGCAGGCGTCCTACGACCTC
CGCCGTGCCCGCGCCGCGGACTGGCCCGCCATCGAGCCGCGCGTCCGCGTGCTCGCCGCAGAGTAG

Structures

Toxin

• AlphaFold DB: A0A2R4GZL3