Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2475705..2476375 Replicon chromosome
Accession NZ_CP089927
Organism Mycobacterium tuberculosis strain M20

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag LV451_RS11820 Protein ID WP_003899954.1
Coordinates 2475705..2476049 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag LV451_RS11825 Protein ID WP_003899955.1
Coordinates 2476046..2476375 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LV451_RS11785 2470778..2471638 + 861 WP_003416628.1 alpha/beta hydrolase -
LV451_RS11790 2471613..2472128 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
LV451_RS11795 2472144..2472355 + 212 Protein_2328 (R)-hydratase -
LV451_RS11800 2472368..2472661 + 294 WP_003416635.1 hypothetical protein -
LV451_RS11805 2472949..2474238 + 1290 WP_003416640.1 ATP-binding protein -
LV451_RS11810 2474585..2475019 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
LV451_RS11815 2475022..2475474 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LV451_RS11820 2475705..2476049 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LV451_RS11825 2476046..2476375 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
LV451_RS11830 2476612..2477873 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
LV451_RS11835 2478095..2479356 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
LV451_RS11840 2479629..2479976 + 348 WP_003899956.1 DUF2384 domain-containing protein -
LV451_RS11845 2479973..2480593 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T229684 WP_003899954.1 NZ_CP089927:2475705-2476049 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T229684 NZ_CP124506:c4499123-4498950 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT229684 WP_003899955.1 NZ_CP089927:2476046-2476375 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT229684 NZ_CP124506:c4499447-4499151 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References