Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 1901889..1902537 Replicon chromosome
Accession NZ_CP089927
Organism Mycobacterium tuberculosis strain M20

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag LV451_RS09035 Protein ID WP_003899414.1
Coordinates 1901889..1902212 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag LV451_RS09040 Protein ID WP_003899415.1
Coordinates 1902292..1902537 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LV451_RS09010 1897463..1898724 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
LV451_RS09015 1898751..1898936 - 186 WP_009935348.1 hypothetical protein -
LV451_RS09020 1899098..1900537 - 1440 WP_009935349.1 phage major capsid protein -
LV451_RS09025 1900545..1901078 - 534 WP_003899412.1 HK97 family phage prohead protease -
LV451_RS09030 1901231..1901722 - 492 WP_003900541.1 phage terminase small subunit P27 family -
LV451_RS09035 1901889..1902212 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
LV451_RS09040 1902292..1902537 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
LV451_RS09045 1902534..1903961 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
LV451_RS09050 1903963..1904355 - 393 WP_003899417.1 DUF2742 domain-containing protein -
LV451_RS09055 1904352..1904612 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
LV451_RS09060 1904629..1904991 - 363 WP_003900543.1 hypothetical protein -
LV451_RS09065 1904994..1906121 - 1128 WP_003899420.1 site-specific integrase -
LV451_RS09070 1906266..1906493 - 228 WP_003899421.1 hypothetical protein -
LV451_RS09075 1906490..1906879 - 390 WP_003899422.1 hypothetical protein -
LV451_RS09080 1906785..1907057 + 273 WP_003900544.1 hypothetical protein -
LV451_RS09085 1907156..1907389 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1895854..1906121 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T229676 WP_003899414.1 NZ_CP089927:c1902212-1901889 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T229676 NZ_CP124506:2920049-2920327 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT229676 WP_003899415.1 NZ_CP089927:c1902537-1902292 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT229676 NZ_CP124506:2919856-2920047 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References