Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 1123391..1124259 Replicon chromosome
Accession NZ_CP089927
Organism Mycobacterium tuberculosis strain M20

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag LV451_RS05220 Protein ID WP_010886136.1
Coordinates 1123391..1123768 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag LV451_RS05225 Protein ID WP_003409886.1
Coordinates 1123810..1124259 (+) Length 150 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LV451_RS05170 1119180..1119632 - 453 WP_003899095.1 lipoprotein -
LV451_RS05175 1119696..1120097 + 402 WP_003409869.1 hypothetical protein -
LV451_RS05180 1120090..1120272 - 183 WP_003409870.1 hypothetical protein -
LV451_RS05185 1120386..1120736 - 351 WP_003899096.1 hypothetical protein -
LV451_RS05190 1120747..1121649 - 903 WP_003899097.1 hypothetical protein -
LV451_RS05195 1121670..1121861 - 192 WP_003409876.1 hypothetical protein -
LV451_RS05200 1121862..1122158 - 297 WP_003409877.1 hypothetical protein -
LV451_RS05205 1122398..1122613 + 216 WP_003409878.1 antitoxin -
LV451_RS05210 1122610..1122921 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
LV451_RS05215 1122895..1123416 - 522 WP_003904745.1 hypothetical protein -
LV451_RS05220 1123391..1123768 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
LV451_RS05225 1123810..1124259 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LV451_RS05230 1124256..1124801 + 546 WP_003409891.1 SecB-like chaperone -
LV451_RS05235 1124690..1125304 - 615 WP_003901296.1 hypothetical protein -
LV451_RS05240 1125353..1125649 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
LV451_RS05245 1125646..1125897 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
LV451_RS05250 1125884..1126378 + 495 WP_003899099.1 hypothetical protein -
LV451_RS05255 1126538..1126945 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
LV451_RS05260 1126949..1127221 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LV451_RS05265 1127254..1128474 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-113)

Antitoxin

(44-91)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T229660 WP_010886136.1 NZ_CP089927:1123391-1123768 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T229660 NZ_CP124502:c4787496-4786969 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT229660 WP_003409886.1 NZ_CP089927:1123810-1124259 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT229660 NZ_CP124502:c4787769-4787503 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU


Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References