Detailed information of TA system TA022962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3005841..3006429
• Replicon: chromosome
• Accession: NC_011004
• Organism: Rhodopseudomonas palustris TIE-1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B3QH83
• Locus tag: RPAL_RS13665
• Protein ID: WP_012495961.1
• Coordinates: 3005841..3006128 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RPAL_RS13670
• Protein ID: WP_012495962.1
• Coordinates: 3006142..3006429 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RPAL_RS13640	3001387..3002892	+	1506	WP_011158030.1	caspase family protein	-
RPAL_RS13645	3002947..3003783	-	837	WP_012495957.1	helix-turn-helix transcriptional regulator	-
RPAL_RS13650	3003882..3004787	+	906	WP_012495958.1	SDR family oxidoreductase	-
RPAL_RS13655	3004821..3005009	-	189	WP_012495959.1	hypothetical protein	-
RPAL_RS13660	3005229..3005699	-	471	WP_012495960.1	hypothetical protein	-
RPAL_RS13665	3005841..3006128	+	288	WP_012495961.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RPAL_RS13670	3006142..3006429	+	288	WP_012495962.1	HigA family addiction module antidote protein	Antitoxin
RPAL_RS13675	3006442..3008493	-	2052	WP_012495963.1	DNA topoisomerase IV subunit B	-
RPAL_RS13680	3008951..3009481	+	531	WP_012495964.1	DedA family protein	-
RPAL_RS13685	3009567..3010556	+	990	WP_012495965.1	MBL fold metallo-hydrolase	-
RPAL_RS13690	3010702..3011247	-	546	WP_012495966.1	DUF2087 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (31-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-59)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11047.69 Da
• Isoelectric Point: 10.2688

>T22962 WP_012495961.1 NC_011004:3005841-3006128 [Rhodopseudomonas palustris TIE-1]
MIRSWRNAATRKLWDGSRFDQFRGLDVEAAIDLLLALNAAKSLQDLSPLQSVGLHKLKGVRRNQWAMTVNDRWRICFEFR
KGDAFEVEIVDYHKG

Nucleotide

• Length: 288 bp

>T22962 NC_011004:3005841-3006128 [Rhodopseudomonas palustris TIE-1]
ATGATCCGGTCATGGCGCAATGCTGCCACCCGGAAGTTATGGGACGGCAGCCGGTTCGACCAATTCCGCGGCCTTGATGT
CGAGGCCGCGATCGATCTGTTGCTGGCTTTGAATGCCGCCAAGTCGCTGCAAGATCTCAGTCCTCTACAGAGCGTAGGAT
TACACAAACTCAAGGGCGTGCGCAGGAACCAATGGGCGATGACGGTCAACGACCGGTGGCGGATTTGTTTCGAGTTTCGC
AAAGGTGATGCGTTCGAAGTCGAGATCGTCGACTATCACAAAGGGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10481.98 Da
• Isoelectric Point: 10.6604

>AT22962 WP_012495962.1 NC_011004:3006142-3006429 [Rhodopseudomonas palustris TIE-1]
MAPVSHPGRLLKRELAARKLSANRLALDIGVPSGRITDILNGRRSITADTAVRLGRYFGNAPQFWLDLQSQYDIAVIERD
KGAEIDRQVRPADAA

Nucleotide

• Length: 288 bp

>AT22962 NC_011004:3006142-3006429 [Rhodopseudomonas palustris TIE-1]
ATGGCTCCTGTTTCCCATCCCGGCCGGCTGCTGAAGCGAGAACTGGCGGCGCGGAAACTCAGCGCCAACCGGCTGGCGCT
GGACATCGGCGTTCCGTCTGGACGGATCACCGACATTCTCAACGGTCGTCGGTCGATTACTGCGGATACCGCGGTGCGGC
TGGGTCGTTATTTCGGAAATGCTCCGCAGTTCTGGCTCGATCTGCAGAGCCAGTACGACATCGCCGTCATCGAGCGTGAC
AAGGGCGCGGAGATTGACCGTCAGGTGCGGCCGGCGGACGCCGCCTGA

Structures

Toxin

• AlphaFold DB: B3QH83