Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3871070..3871667 Replicon chromosome
Accession NZ_CP089884
Organism Klebsiella pneumoniae strain KP102

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LR951_RS18935 Protein ID WP_004142563.1
Coordinates 3871350..3871667 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LR951_RS18930 Protein ID WP_004142561.1
Coordinates 3871070..3871357 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LR951_RS18900 (LR951_18870) 3867277..3867525 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LR951_RS18905 (LR951_18875) 3867543..3867884 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LR951_RS18910 (LR951_18880) 3867915..3869030 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
LR951_RS18915 (LR951_18885) 3869210..3869791 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
LR951_RS18920 (LR951_18890) 3869791..3870159 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LR951_RS18925 (LR951_18895) 3870279..3870932 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
LR951_RS18930 (LR951_18900) 3871070..3871357 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LR951_RS18935 (LR951_18905) 3871350..3871667 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LR951_RS18940 (LR951_18910) 3871852..3872895 - 1044 WP_020802450.1 DUF2157 domain-containing protein -
LR951_RS18945 (LR951_18915) 3873562..3874428 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
LR951_RS18950 (LR951_18920) 3874537..3875964 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T229604 WP_004142563.1 NZ_CP089884:c3871667-3871350 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T229604 NZ_CP124498:1014328-1014735 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT229604 WP_004142561.1 NZ_CP089884:c3871357-3871070 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT229604 NZ_CP124498:1014081-1014347 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References