Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 323019..323605 Replicon chromosome
Accession NZ_CP089882
Organism Klebsiella pneumoniae strain KP059

Toxin (Protein)


Gene name doc Uniprot ID A0A486Q6W0
Locus tag LVQ69_RS01500 Protein ID WP_020802489.1
Coordinates 323237..323605 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag LVQ69_RS01495 Protein ID WP_004174006.1
Coordinates 323019..323240 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ69_RS01475 (LVQ69_01475) 319176..320102 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LVQ69_RS01480 (LVQ69_01480) 320099..321376 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
LVQ69_RS01485 (LVQ69_01485) 321373..322140 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LVQ69_RS01490 (LVQ69_01490) 322142..322855 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LVQ69_RS01495 (LVQ69_01495) 323019..323240 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVQ69_RS01500 (LVQ69_01500) 323237..323605 + 369 WP_020802489.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LVQ69_RS01505 (LVQ69_01505) 323878..325194 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LVQ69_RS01510 (LVQ69_01510) 325301..326188 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LVQ69_RS01515 (LVQ69_01515) 326185..327030 + 846 WP_023328323.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LVQ69_RS01520 (LVQ69_01520) 327032..328102 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 320099..328839 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13542.89 Da        Isoelectric Point: 8.6410

>T229572 WP_020802489.1 NZ_CP089882:323237-323605 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTSGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T229572 NZ_CP124492:c4355117-4354944 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT229572 WP_004174006.1 NZ_CP089882:323019-323240 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT229572 NZ_CP124492:c4355441-4355145 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486Q6W0

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References