Detailed information of TA system TA229552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 353469..354115
• Replicon: chromosome
• Accession: NZ_CP089880
• Organism: Klebsiella pneumoniae strain KP309

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: LVQ72_RS01640
• Protein ID: WP_016529833.1
• Coordinates: 353469..353816 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LVQ72_RS01645
• Protein ID: WP_048255315.1
• Coordinates: 353816..354115 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVQ72_RS01630 (LVQ72_01630)	349395..350828	+	1434	WP_002920564.1	glycogen synthase GlgA	-
LVQ72_RS01635 (LVQ72_01635)	350846..353293	+	2448	WP_002920561.1	glycogen phosphorylase	-
LVQ72_RS01640 (LVQ72_01640)	353469..353816	+	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVQ72_RS01645 (LVQ72_01645)	353816..354115	+	300	WP_048255315.1	helix-turn-helix transcriptional regulator	Antitoxin
LVQ72_RS01650 (LVQ72_01650)	354178..355686	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
LVQ72_RS01655 (LVQ72_01655)	355891..356220	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
LVQ72_RS01660 (LVQ72_01660)	356271..357101	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
LVQ72_RS01665 (LVQ72_01665)	357151..357909	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T229552 WP_016529833.1 NZ_CP089880:353469-353816 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T229552 NZ_CP124491:c138677-138372 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10858.66 Da
• Isoelectric Point: 10.1464

>AT229552 WP_048255315.1 NZ_CP089880:353816-354115 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAVKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT229552 NZ_CP124491:c138897-138679 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: W9BBY1