Detailed information of TA system TA229549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 247694..248351
• Replicon: plasmid pKP308-1
• Accession: NZ_CP089878
• Organism: Klebsiella pneumoniae strain KP308

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: LVQ70_RS27090
• Protein ID: WP_000270043.1
• Coordinates: 247694..248044 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LVQ70_RS27095
• Protein ID: WP_000124640.1
• Coordinates: 248049..248351 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVQ70_RS27055 (LVQ70_27025)	244168..244665	-	498	WP_000062185.1	hypothetical protein	-
LVQ70_RS27060 (LVQ70_27030)	244668..245156	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
LVQ70_RS27065 (LVQ70_27035)	245253..245588	+	336	WP_000683476.1	hypothetical protein	-
LVQ70_RS27070 (LVQ70_27040)	245603..246073	-	471	WP_001281821.1	hypothetical protein	-
LVQ70_RS27075 (LVQ70_27045)	246066..246437	-	372	WP_000516916.1	hypothetical protein	-
LVQ70_RS27080 (LVQ70_27050)	246448..246642	-	195	WP_000343597.1	hypothetical protein	-
LVQ70_RS27085 (LVQ70_27055)	246983..247531	-	549	WP_001061574.1	transcriptional regulator	-
LVQ70_RS27090 (LVQ70_27060)	247694..248044	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVQ70_RS27095 (LVQ70_27065)	248049..248351	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
LVQ70_RS27100 (LVQ70_27070)	248378..248671	-	294	WP_001239997.1	chromosome segregation protein ParM	-
LVQ70_RS27105 (LVQ70_27075)	248759..249031	-	273	WP_001043047.1	HU family DNA-binding protein	-
LVQ70_RS27110 (LVQ70_27080)	249089..249616	-	528	WP_001236377.1	thermonuclease family protein	-
LVQ70_RS27115 (LVQ70_27085)	249847..250704	-	858	WP_001167032.1	hypothetical protein	-
LVQ70_RS27120 (LVQ70_27090)	250691..250921	-	231	WP_000972663.1	hypothetical protein	-
LVQ70_RS27125 (LVQ70_27095)	250921..251439	-	519	WP_000210756.1	nitrite reductase	-
LVQ70_RS27130 (LVQ70_27100)	251436..251882	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
LVQ70_RS27135 (LVQ70_27105)	251882..252241	-	360	WP_000422768.1	hypothetical protein	-
LVQ70_RS27140 (LVQ70_27110)	252298..252726	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / qnrS1 / sul2 / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / tet(D) / aph(3')-Ia	-	1..309645	309645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T229549 WP_000270043.1 NZ_CP089878:247694-248044 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T229549 NZ_CP124490:3949187-3949321 [Escherichia coli]
ATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCA
CATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT229549 WP_000124640.1 NZ_CP089878:248049-248351 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT229549 NZ_CP124490:c3949120-3949063 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9