Detailed information of TA system TA229528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 102741..103468
• Replicon: plasmid pKP106-1
• Accession: NZ_CP089875
• Organism: Klebsiella pneumoniae strain KP106

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LVQ73_RS25905
• Protein ID: WP_011251285.1
• Coordinates: 102741..103052 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LVQ73_RS25910
• Protein ID: WP_011251286.1
• Coordinates: 103049..103468 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVQ73_RS25875 (LVQ73_25850)	97971..98951	+	981	WP_048293850.1	IS5-like element ISKpn26 family transposase	-
LVQ73_RS25880 (LVQ73_25855)	99497..99676	-	180	Protein_101	IS5/IS1182 family transposase	-
LVQ73_RS25885 (LVQ73_25860)	99716..101254	-	1539	WP_032430751.1	IS66 family transposase	-
LVQ73_RS25890 (LVQ73_25865)	101303..101650	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
LVQ73_RS25895 (LVQ73_25870)	101647..101985	-	339	WP_023288266.1	transposase	-
LVQ73_RS25900 (LVQ73_25875)	102099..102536	+	438	Protein_105	DDE-type integrase/transposase/recombinase	-
LVQ73_RS25905 (LVQ73_25880)	102741..103052	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LVQ73_RS25910 (LVQ73_25885)	103049..103468	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LVQ73_RS25915 (LVQ73_25890)	103538..103840	+	303	WP_167813119.1	hypothetical protein	-
LVQ73_RS25920 (LVQ73_25895)	103888..104001	-	114	Protein_109	hypothetical protein	-
LVQ73_RS25925 (LVQ73_25900)	104046..105014	-	969	WP_167813116.1	IS5-like element IS903B family transposase	-
LVQ73_RS25930 (LVQ73_25905)	105047..105934	-	888	Protein_111	IS5-like element ISKpn26 family transposase	-
LVQ73_RS25935 (LVQ73_25910)	105936..106481	+	546	Protein_112	methyl-accepting chemotaxis protein	-
LVQ73_RS25940 (LVQ73_25915)	107057..107263	+	207	Protein_113	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib	cheD	1..186670	186670
	-	inside	IScluster/Tn	-	cheD	97971..109855	11884

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T229528 WP_011251285.1 NZ_CP089875:102741-103052 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T229528 NZ_CP124487:c4047944-4047630 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT229528 WP_011251286.1 NZ_CP089875:103049-103468 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT229528 NZ_CP124487:c4048180-4047947 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1