Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 1381166..1381730 Replicon chromosome
Accession NC_010999
Organism Lactobacillus casei BL23

Toxin (Protein)


Gene name yafQ Uniprot ID A0A6G9EML4
Locus tag LCABL_RS07125 Protein ID WP_014951859.1
Coordinates 1381428..1381730 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag LCABL_RS07120 Protein ID WP_003570051.1
Coordinates 1381166..1381435 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCABL_RS07090 1376170..1377057 - 888 WP_012491506.1 ABC transporter ATP-binding protein -
LCABL_RS07095 1377255..1378481 - 1227 WP_012491507.1 hypothetical protein -
LCABL_RS07100 1378679..1379083 + 405 WP_012491508.1 MerR family transcriptional regulator -
LCABL_RS07105 1379076..1379402 + 327 WP_012491509.1 YrdB family protein -
LCABL_RS07110 1379465..1380094 + 630 WP_012491510.1 DUF2399 domain-containing protein -
LCABL_RS07115 1380139..1380792 + 654 WP_012491511.1 GNAT family N-acetyltransferase -
LCABL_RS07120 1381166..1381435 + 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
LCABL_RS07125 1381428..1381730 + 303 WP_014951859.1 type II toxin-antitoxin system YafQ family toxin Toxin
LCABL_RS16905 1382069..1382212 + 144 WP_003574774.1 hypothetical protein -
LCABL_RS07130 1382330..1383022 + 693 WP_012491513.1 hypothetical protein -
LCABL_RS07135 1383258..1385435 + 2178 WP_012491514.1 RNA-binding transcriptional accessory protein -
LCABL_RS07140 1385500..1386429 - 930 WP_003599410.1 IS30-like element ISLpl1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1385500..1386429 929


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-98)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11441.27 Da        Isoelectric Point: 10.2818

>T22952 WP_014951859.1 NC_010999:1381428-1381730 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIK
QQDLILTLVRTGSHHNLLGK

Download         Length: 303 bp

>T22952 NC_010999:1381428-1381730 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCTGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCTT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTAAG
CAGCAAGATCTCATTTTGACCCTGGTTAGAACTGGATCTCATCATAACCTTTTGGGTAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT22952 WP_003570051.1 NC_010999:1381166-1381435 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT22952 NC_010999:1381166-1381435 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTGTTGATCCGGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACTATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G9EML4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References