Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 61790..62315 Replicon plasmid pA
Accession NC_010998
Organism Rhizobium etli CIAT 652

Toxin (Protein)


Gene name yoeB Uniprot ID B3Q138
Locus tag RHECIAT_RS21925 Protein ID WP_010025739.1
Coordinates 62043..62315 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A192TIM2
Locus tag RHECIAT_RS21920 Protein ID WP_010025738.1
Coordinates 61790..62041 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RHECIAT_RS21905 58530..59873 - 1344 WP_004679763.1 adenylate/guanylate cyclase domain-containing protein -
RHECIAT_RS21915 60373..61710 + 1338 WP_041683963.1 nucleotide sugar dehydrogenase -
RHECIAT_RS21920 61790..62041 + 252 WP_010025738.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
RHECIAT_RS21925 62043..62315 + 273 WP_010025739.1 Txe/YoeB family addiction module toxin Toxin
RHECIAT_RS21930 62353..63528 - 1176 WP_012490438.1 DUF3095 domain-containing protein -
RHECIAT_RS21935 63651..64130 + 480 WP_012490439.1 class I SAM-dependent methyltransferase -
RHECIAT_RS32245 64201..64737 + 537 WP_139052609.1 hypothetical protein -
RHECIAT_RS21940 64815..66728 - 1914 WP_012490440.1 adenylate/guanylate cyclase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..414090 414090


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10743.42 Da        Isoelectric Point: 9.4530

>T22949 WP_010025739.1 NC_010998:62043-62315 [Rhizobium etli CIAT 652]
MKLLWTPTSWEEYQYWQKTDQKMVEKINELLKDTKRSPFKGLGKPEPLKGDLSGLWSRRILGEHRLVYCVTGKGSDQQLE
IIQCRFHYEK

Download         Length: 273 bp

>T22949 NC_010998:62043-62315 [Rhizobium etli CIAT 652]
ATGAAGCTGCTATGGACCCCGACTTCCTGGGAAGAATACCAATACTGGCAGAAAACCGACCAGAAGATGGTCGAGAAAAT
CAACGAACTGCTCAAGGATACAAAAAGATCGCCCTTTAAAGGTCTCGGCAAGCCGGAACCGCTGAAGGGCGATCTATCCG
GACTTTGGTCCCGGCGTATCCTCGGAGAGCATCGACTCGTCTACTGCGTTACTGGCAAGGGTTCAGACCAGCAGTTGGAA
ATCATCCAATGCCGATTTCATTACGAAAAATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9345.63 Da        Isoelectric Point: 5.6513

>AT22949 WP_010025738.1 NC_010998:61790-62041 [Rhizobium etli CIAT 652]
MAHVRLTEFRQNIATYFDQVLSSRAPLLVTRQGSEAMVLIAESEYESMQETLHLLSTPANSARLRESIAQLRKGQVIESD
PTL

Download         Length: 252 bp

>AT22949 NC_010998:61790-62041 [Rhizobium etli CIAT 652]
ATGGCACACGTCCGATTGACGGAGTTCCGGCAGAACATCGCAACCTATTTTGATCAGGTCCTGTCTTCTCGGGCTCCTCT
GCTCGTGACTCGTCAAGGTTCGGAAGCAATGGTCTTGATTGCCGAAAGCGAGTACGAAAGCATGCAAGAGACGCTGCACC
TGCTCTCGACGCCTGCCAATTCCGCCCGCTTGCGTGAAAGCATCGCACAGCTTCGCAAGGGCCAGGTGATAGAGAGCGAC
CCGACTCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192TIV7

Antitoxin

Source ID Structure
AlphaFold DB A0A192TIM2

References