Detailed information of TA system TA229487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5436162..5436748
• Replicon: chromosome
• Accession: NZ_CP089849
• Organism: Pseudomonas aeruginosa strain PA0523

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LVE67_RS25785
• Protein ID: WP_003111576.1
• Coordinates: 5436479..5436748 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0S3KUN4
• Locus tag: LVE67_RS25780
• Protein ID: WP_003111575.1
• Coordinates: 5436162..5436467 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVE67_RS25750 (LVE67_25750)	5431456..5431728	-	273	WP_003115921.1	hypothetical protein	-
LVE67_RS25755 (LVE67_25755)	5432316..5432510	+	195	WP_003111573.1	hypothetical protein	-
LVE67_RS25760 (LVE67_25760)	5432630..5433256	-	627	WP_232772639.1	hypothetical protein	-
LVE67_RS25765 (LVE67_25765)	5433285..5434313	+	1029	WP_034064933.1	IS30-like element ISPsp7 family transposase	-
LVE67_RS25770 (LVE67_25770)	5434325..5435179	-	855	WP_232772640.1	hypothetical protein	-
LVE67_RS25775 (LVE67_25775)	5435442..5435825	-	384	WP_003111574.1	hypothetical protein	-
LVE67_RS25780 (LVE67_25780)	5436162..5436467	-	306	WP_003111575.1	HigA family addiction module antitoxin	Antitoxin
LVE67_RS25785 (LVE67_25785)	5436479..5436748	-	270	WP_003111576.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVE67_RS25790 (LVE67_25790)	5436801..5436929	-	129	Protein_5105	integrase	-
LVE67_RS25795 (LVE67_25795)	5437077..5439305	+	2229	WP_023443586.1	TonB-dependent receptor	-
LVE67_RS25800 (LVE67_25800)	5439376..5440023	-	648	WP_003095021.1	carbonate dehydratase	-
LVE67_RS25805 (LVE67_25805)	5440085..5441323	-	1239	WP_003111578.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5433285..5434313	1028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-89)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10330.76 Da
• Isoelectric Point: 7.8937

>T229487 WP_003111576.1 NZ_CP089849:c5436748-5436479 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTDAGP
EEVEIVDYH

Nucleotide

• Length: 270 bp

>T229487 NZ_CP124482:99864-100244 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11205.72 Da
• Isoelectric Point: 4.4475

>AT229487 WP_003111575.1 NZ_CP089849:c5436467-5436162 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT229487 NZ_CP124482:99643-99864 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3KUN4