Detailed information of TA system TA229473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4606375..4606977
• Replicon: chromosome
• Accession: NZ_CP089799
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain ZTA19/00785

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: LUY21_RS22235
• Protein ID: WP_023138876.1
• Coordinates: 4606666..4606977 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LUY21_RS22230
• Protein ID: WP_000362052.1
• Coordinates: 4606375..4606665 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUY21_RS22215 (4603868)	4603868..4604770	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
LUY21_RS22220 (4604767)	4604767..4605402	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LUY21_RS22225 (4605399)	4605399..4606328	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LUY21_RS22230 (4606375)	4606375..4606665	-	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
LUY21_RS22235 (4606666)	4606666..4606977	-	312	WP_023138876.1	hypothetical protein	Toxin
LUY21_RS22240 (4607195)	4607195..4608124	+	930	WP_001127698.1	alpha/beta hydrolase	-
LUY21_RS22245 (4608210)	4608210..4608521	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LUY21_RS22250 (4608518)	4608518..4608964	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LUY21_RS22255 (4608979)	4608979..4609920	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LUY21_RS22260 (4609965)	4609965..4610402	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
LUY21_RS22265 (4610399)	4610399..4611271	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LUY21_RS22270 (4611265)	4611265..4611864	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T229473 WP_023138876.1 NZ_CP089799:c4606977-4606666 [Salmonella enterica subsp. enterica serovar Kentucky]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T229473 NZ_CP124481:c2126597-2126319 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT229473 WP_000362052.1 NZ_CP089799:c4606665-4606375 [Salmonella enterica subsp. enterica serovar Kentucky]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT229473 NZ_CP124481:c2126790-2126599 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A603E470