Detailed information of TA system TA229452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4607012..4607614
• Replicon: chromosome
• Accession: NZ_CP089798
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain ZTA19/00789

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: LUY14_RS22230
• Protein ID: WP_023138876.1
• Coordinates: 4607303..4607614 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LUY14_RS22225
• Protein ID: WP_000362052.1
• Coordinates: 4607012..4607302 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUY14_RS22210 (4604505)	4604505..4605407	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
LUY14_RS22215 (4605404)	4605404..4606039	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LUY14_RS22220 (4606036)	4606036..4606965	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LUY14_RS22225 (4607012)	4607012..4607302	-	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
LUY14_RS22230 (4607303)	4607303..4607614	-	312	WP_023138876.1	hypothetical protein	Toxin
LUY14_RS22235 (4607832)	4607832..4608761	+	930	WP_001127698.1	alpha/beta hydrolase	-
LUY14_RS22240 (4608847)	4608847..4609158	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LUY14_RS22245 (4609155)	4609155..4609601	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LUY14_RS22250 (4609616)	4609616..4610557	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LUY14_RS22255 (4610602)	4610602..4611039	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
LUY14_RS22260 (4611036)	4611036..4611908	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LUY14_RS22265 (4611902)	4611902..4612501	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T229452 WP_023138876.1 NZ_CP089798:c4607614-4607303 [Salmonella enterica subsp. enterica serovar Kentucky]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T229452 NZ_CP124475:c3700270-3699968 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT229452 WP_000362052.1 NZ_CP089798:c4607302-4607012 [Salmonella enterica subsp. enterica serovar Kentucky]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT229452 NZ_CP124475:c3699932-3699591 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A603E470