Detailed information of TA system TA229410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4649758..4650360
• Replicon: chromosome
• Accession: NZ_CP089795
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain ZTA19/00813

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: LUY22_RS22505
• Protein ID: WP_023138876.1
• Coordinates: 4650049..4650360 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LUY22_RS22500
• Protein ID: WP_000362052.1
• Coordinates: 4649758..4650048 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUY22_RS22485 (4647251)	4647251..4648153	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
LUY22_RS22490 (4648150)	4648150..4648785	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LUY22_RS22495 (4648782)	4648782..4649711	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LUY22_RS22500 (4649758)	4649758..4650048	-	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
LUY22_RS22505 (4650049)	4650049..4650360	-	312	WP_023138876.1	hypothetical protein	Toxin
LUY22_RS22510 (4650578)	4650578..4651507	+	930	WP_001127698.1	alpha/beta hydrolase	-
LUY22_RS22515 (4651593)	4651593..4651904	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LUY22_RS22520 (4651901)	4651901..4652347	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LUY22_RS22525 (4652362)	4652362..4653303	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LUY22_RS22530 (4653348)	4653348..4653785	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
LUY22_RS22535 (4653782)	4653782..4654654	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LUY22_RS22540 (4654648)	4654648..4655247	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T229410 WP_023138876.1 NZ_CP089795:c4650360-4650049 [Salmonella enterica subsp. enterica serovar Kentucky]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T229410 NZ_CP124467:c3907983-3907699 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT229410 WP_000362052.1 NZ_CP089795:c4650048-4649758 [Salmonella enterica subsp. enterica serovar Kentucky]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT229410 NZ_CP124467:c3908219-3907986 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A603E470