Detailed information of TA system TA229389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4606365..4606967
• Replicon: chromosome
• Accession: NZ_CP089794
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain ZTA19/00814

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: LUY18_RS22215
• Protein ID: WP_023138876.1
• Coordinates: 4606656..4606967 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LUY18_RS22210
• Protein ID: WP_000362052.1
• Coordinates: 4606365..4606655 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUY18_RS22195 (4603858)	4603858..4604760	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
LUY18_RS22200 (4604757)	4604757..4605392	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LUY18_RS22205 (4605389)	4605389..4606318	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LUY18_RS22210 (4606365)	4606365..4606655	-	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
LUY18_RS22215 (4606656)	4606656..4606967	-	312	WP_023138876.1	hypothetical protein	Toxin
LUY18_RS22220 (4607185)	4607185..4608114	+	930	WP_001127698.1	alpha/beta hydrolase	-
LUY18_RS22225 (4608200)	4608200..4608511	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LUY18_RS22230 (4608508)	4608508..4608954	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LUY18_RS22235 (4608969)	4608969..4609910	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LUY18_RS22240 (4609955)	4609955..4610392	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
LUY18_RS22245 (4610389)	4610389..4611261	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LUY18_RS22250 (4611255)	4611255..4611854	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T229389 WP_023138876.1 NZ_CP089794:c4606967-4606656 [Salmonella enterica subsp. enterica serovar Kentucky]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T229389 NZ_CP124461:44289-44594 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT229389 WP_000362052.1 NZ_CP089794:c4606655-4606365 [Salmonella enterica subsp. enterica serovar Kentucky]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT229389 NZ_CP124461:44069-44287 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A603E470