Detailed information of TA system TA229347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4605646..4606248
• Replicon: chromosome
• Accession: NZ_CP089791
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain ZTA19/00820

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: LUY13_RS22220
• Protein ID: WP_023138876.1
• Coordinates: 4605937..4606248 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LUY13_RS22215
• Protein ID: WP_000362052.1
• Coordinates: 4605646..4605936 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUY13_RS22200 (4603139)	4603139..4604041	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
LUY13_RS22205 (4604038)	4604038..4604673	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LUY13_RS22210 (4604670)	4604670..4605599	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LUY13_RS22215 (4605646)	4605646..4605936	-	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
LUY13_RS22220 (4605937)	4605937..4606248	-	312	WP_023138876.1	hypothetical protein	Toxin
LUY13_RS22225 (4606466)	4606466..4607395	+	930	WP_001127698.1	alpha/beta hydrolase	-
LUY13_RS22230 (4607481)	4607481..4607792	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LUY13_RS22235 (4607789)	4607789..4608235	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LUY13_RS22240 (4608250)	4608250..4609191	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LUY13_RS22245 (4609236)	4609236..4609673	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
LUY13_RS22250 (4609670)	4609670..4610542	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LUY13_RS22255 (4610536)	4610536..4611135	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T229347 WP_023138876.1 NZ_CP089791:c4606248-4605937 [Salmonella enterica subsp. enterica serovar Kentucky]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T229347 NZ_CP124455:606986-607273 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT229347 WP_000362052.1 NZ_CP089791:c4605936-4605646 [Salmonella enterica subsp. enterica serovar Kentucky]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT229347 NZ_CP124455:607260-607562 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A603E470