Detailed information of TA system TA229326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4605867..4606469
• Replicon: chromosome
• Accession: NZ_CP089790
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain ZTA19/00830

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: LUY12_RS22205
• Protein ID: WP_023138876.1
• Coordinates: 4606158..4606469 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LUY12_RS22200
• Protein ID: WP_000362052.1
• Coordinates: 4605867..4606157 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUY12_RS22185 (4603360)	4603360..4604262	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
LUY12_RS22190 (4604259)	4604259..4604894	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LUY12_RS22195 (4604891)	4604891..4605820	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LUY12_RS22200 (4605867)	4605867..4606157	-	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
LUY12_RS22205 (4606158)	4606158..4606469	-	312	WP_023138876.1	hypothetical protein	Toxin
LUY12_RS22210 (4606687)	4606687..4607616	+	930	WP_001127698.1	alpha/beta hydrolase	-
LUY12_RS22215 (4607702)	4607702..4608013	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LUY12_RS22220 (4608010)	4608010..4608456	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LUY12_RS22225 (4608471)	4608471..4609412	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LUY12_RS22230 (4609457)	4609457..4609894	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
LUY12_RS22235 (4609891)	4609891..4610763	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LUY12_RS22240 (4610757)	4610757..4611356	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T229326 WP_023138876.1 NZ_CP089790:c4606469-4606158 [Salmonella enterica subsp. enterica serovar Kentucky]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T229326 NZ_CP124442:c2162710-2162396 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT229326 WP_000362052.1 NZ_CP089790:c4606157-4605867 [Salmonella enterica subsp. enterica serovar Kentucky]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT229326 NZ_CP124442:c2162399-2161983 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A603E470