Detailed information of TA system TA229266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 279002..279604
• Replicon: chromosome
• Accession: NZ_CP089788
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain ZTA19/00847

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: LUY19_RS01380
• Protein ID: WP_023138876.1
• Coordinates: 279002..279313 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LUY19_RS01385
• Protein ID: WP_000362052.1
• Coordinates: 279314..279604 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUY19_RS01345 (274115)	274115..274714	+	600	WP_000965695.1	glucose-1-phosphatase	-
LUY19_RS01350 (274708)	274708..275580	+	873	WP_000921423.1	virulence factor BrkB family protein	-
LUY19_RS01355 (275577)	275577..276014	+	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
LUY19_RS01360 (276059)	276059..277000	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LUY19_RS01365 (277015)	277015..277461	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LUY19_RS01370 (277458)	277458..277769	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LUY19_RS01375 (277855)	277855..278784	-	930	WP_001127698.1	alpha/beta hydrolase	-
LUY19_RS01380 (279002)	279002..279313	+	312	WP_023138876.1	hypothetical protein	Toxin
LUY19_RS01385 (279314)	279314..279604	+	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
LUY19_RS01390 (279651)	279651..280580	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LUY19_RS01395 (280577)	280577..281212	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LUY19_RS01400 (281209)	281209..282111	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T229266 WP_023138876.1 NZ_CP089788:279002-279313 [Salmonella enterica subsp. enterica serovar Kentucky]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T229266 NZ_CP124425:c90095-89790 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT229266 WP_000362052.1 NZ_CP089788:279314-279604 [Salmonella enterica subsp. enterica serovar Kentucky]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT229266 NZ_CP124425:c90315-90097 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A603E470