Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3538901..3539571 Replicon chromosome
Accession NZ_CP089781
Organism Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag LVJ76_RS16775 Protein ID WP_003899954.1
Coordinates 3538901..3539245 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag LVJ76_RS16780 Protein ID WP_003899955.1
Coordinates 3539242..3539571 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ76_RS16740 (LVJ76_16755) 3533974..3534834 + 861 WP_003416628.1 alpha/beta hydrolase -
LVJ76_RS16745 (LVJ76_16760) 3534809..3535324 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
LVJ76_RS16750 (LVJ76_16765) 3535340..3535551 + 212 Protein_3307 (R)-hydratase -
LVJ76_RS16755 (LVJ76_16770) 3535564..3535857 + 294 WP_003416635.1 hypothetical protein -
LVJ76_RS16760 (LVJ76_16775) 3536145..3537434 + 1290 WP_003906996.1 ATP-binding protein -
LVJ76_RS16765 (LVJ76_16780) 3537781..3538215 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
LVJ76_RS16770 (LVJ76_16785) 3538218..3538670 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ76_RS16775 (LVJ76_16790) 3538901..3539245 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ76_RS16780 (LVJ76_16795) 3539242..3539571 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
LVJ76_RS16785 (LVJ76_16800) 3540109..3540456 + 348 WP_003899956.1 DUF2384 domain-containing protein -
LVJ76_RS16790 (LVJ76_16805) 3540453..3541073 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
LVJ76_RS16795 (LVJ76_16810) 3541233..3542498 - 1266 WP_023641391.1 hypothetical protein -
LVJ76_RS16800 (LVJ76_16815) 3542666..3542875 + 210 WP_003416778.1 hypothetical protein -
LVJ76_RS16805 (LVJ76_16820) 3543122..3544156 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T229260 WP_003899954.1 NZ_CP089781:3538901-3539245 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T229260 NZ_CP124424:3623896-3624180 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT229260 WP_003899955.1 NZ_CP089781:3539242-3539571 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT229260 NZ_CP124424:3623660-3623893 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References