Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2251486..2252405 Replicon chromosome
Accession NZ_CP089781
Organism Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)


Gene name HigB2 Uniprot ID A0A654U351
Locus tag LVJ76_RS10575 Protein ID WP_003906719.1
Coordinates 2251800..2252405 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag LVJ76_RS10570 Protein ID WP_003410124.1
Coordinates 2251486..2251791 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ76_RS10540 (LVJ76_10555) 2246497..2247753 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
LVJ76_RS10545 (LVJ76_10560) 2248107..2248682 + 576 WP_003410100.1 hypothetical protein -
LVJ76_RS10550 (LVJ76_10565) 2248679..2249719 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
LVJ76_RS10555 (LVJ76_10570) 2249961..2250680 + 720 WP_003410108.1 DUF433 domain-containing protein -
LVJ76_RS10560 (LVJ76_10575) 2250670..2251086 + 417 WP_003410114.1 hypothetical protein -
LVJ76_RS10565 (LVJ76_10580) 2251102..2251401 - 300 WP_003410120.1 hypothetical protein -
LVJ76_RS10570 (LVJ76_10585) 2251486..2251791 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
LVJ76_RS10575 (LVJ76_10590) 2251800..2252405 - 606 WP_003906719.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ76_RS10580 (LVJ76_10595) 2252430..2252789 - 360 WP_003410131.1 hypothetical protein -
LVJ76_RS10585 (LVJ76_10600) 2252949..2253539 - 591 WP_057145698.1 hypothetical protein -
LVJ76_RS10590 (LVJ76_10605) 2253578..2253814 - 237 WP_003410133.1 hypothetical protein -
LVJ76_RS10595 (LVJ76_10610) 2254021..2254917 - 897 WP_003906720.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2244067..2254917 10850


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22716.91 Da        Isoelectric Point: 7.3073

>T229242 WP_003906719.1 NZ_CP089781:c2252405-2251800 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T229242 NZ_CP124421:118535-118915 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT229242 WP_003410124.1 NZ_CP089781:c2251791-2251486 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT229242 NZ_CP124421:118314-118535 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A654U351

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References