Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2261841..2262760 Replicon chromosome
Accession NZ_CP089780
Organism Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag LVJ75_RS10630 Protein ID WP_003900449.1
Coordinates 2262155..2262760 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag LVJ75_RS10625 Protein ID WP_003410124.1
Coordinates 2261841..2262146 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ75_RS10590 (LVJ75_10585) 2257301..2257678 + 378 Protein_2093 hypothetical protein -
LVJ75_RS10595 (LVJ75_10590) 2257675..2257929 + 255 Protein_2094 helix-turn-helix transcriptional regulator -
LVJ75_RS10605 (LVJ75_10600) 2259283..2260074 + 792 Protein_2096 ImmA/IrrE family metallo-endopeptidase -
LVJ75_RS10610 (LVJ75_10605) 2260316..2261035 + 720 WP_003410108.1 DUF433 domain-containing protein -
LVJ75_RS10615 (LVJ75_10610) 2261025..2261441 + 417 WP_003410114.1 hypothetical protein -
LVJ75_RS10620 (LVJ75_10615) 2261457..2261756 - 300 WP_003410120.1 hypothetical protein -
LVJ75_RS10625 (LVJ75_10620) 2261841..2262146 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
LVJ75_RS10630 (LVJ75_10625) 2262155..2262760 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ75_RS10635 (LVJ75_10630) 2262785..2263144 - 360 WP_003410131.1 hypothetical protein -
LVJ75_RS10640 (LVJ75_10635) 2263304..2263894 - 591 WP_223695536.1 hypothetical protein -
LVJ75_RS10645 (LVJ75_10640) 2263933..2264169 - 237 WP_003410133.1 hypothetical protein -
LVJ75_RS10650 (LVJ75_10645) 2264376..2265272 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2255989..2271745 15756


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T229181 WP_003900449.1 NZ_CP089780:c2262760-2262155 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T229181 NZ_CP124410:3331155-3331505 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT229181 WP_003410124.1 NZ_CP089780:c2262146-2261841 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT229181 NZ_CP124410:3330910-3331161 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References