Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1993846..1994765 Replicon chromosome
Accession NZ_CP089779
Organism Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag LVJ74_RS09385 Protein ID WP_003900449.1
Coordinates 1993846..1994451 (+) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag LVJ74_RS09390 Protein ID WP_003410124.1
Coordinates 1994460..1994765 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ74_RS09365 (LVJ74_09345) 1991334..1992230 + 897 WP_003410136.1 hypothetical protein -
LVJ74_RS09370 (LVJ74_09350) 1992437..1992673 + 237 WP_003410133.1 hypothetical protein -
LVJ74_RS09375 (LVJ74_09355) 1992712..1993302 + 591 WP_057145698.1 hypothetical protein -
LVJ74_RS09380 (LVJ74_09360) 1993462..1993821 + 360 WP_003410131.1 hypothetical protein -
LVJ74_RS09385 (LVJ74_09365) 1993846..1994451 + 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ74_RS09390 (LVJ74_09370) 1994460..1994765 + 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
LVJ74_RS09395 (LVJ74_09375) 1994850..1995149 + 300 WP_003410120.1 hypothetical protein -
LVJ74_RS09400 (LVJ74_09380) 1995165..1995581 - 417 WP_003410114.1 hypothetical protein -
LVJ74_RS09405 (LVJ74_09385) 1995571..1996290 - 720 WP_003410108.1 DUF433 domain-containing protein -
LVJ74_RS09410 (LVJ74_09390) 1996532..1997572 - 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
LVJ74_RS09415 (LVJ74_09395) 1997569..1998144 - 576 WP_003410100.1 hypothetical protein -
LVJ74_RS09420 (LVJ74_09400) 1998498..1999754 + 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T229105 WP_003900449.1 NZ_CP089779:1993846-1994451 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T229105 NZ_CP124387:3378734-3379084 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT229105 WP_003410124.1 NZ_CP089779:1994460-1994765 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT229105 NZ_CP124387:3378489-3378740 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References