229087

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 711669..712339 Replicon chromosome
Accession NZ_CP089779
Organism Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag LVJ74_RS03230 Protein ID WP_003899954.1
Coordinates 711995..712339 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag LVJ74_RS03225 Protein ID WP_003899955.1
Coordinates 711669..711998 (-) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ74_RS03200 (LVJ74_03185) 707007..707216 - 210 WP_003416778.1 hypothetical protein -
LVJ74_RS03205 (LVJ74_03190) 707384..708649 + 1266 WP_003912135.1 hypothetical protein -
LVJ74_RS03210 (LVJ74_03195) 708783..710044 + 1262 WP_096874057.1 IS3-like element IS987 family transposase -
LVJ74_RS03215 (LVJ74_03200) 710167..710787 - 621 WP_003899957.1 RES family NAD+ phosphorylase -
LVJ74_RS03220 (LVJ74_03205) 710784..711131 - 348 WP_003899956.1 DUF2384 domain-containing protein -
LVJ74_RS03225 (LVJ74_03210) 711669..711998 - 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
LVJ74_RS03230 (LVJ74_03215) 711995..712339 - 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ74_RS03235 (LVJ74_03220) 712570..713022 + 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ74_RS03240 (LVJ74_03225) 713025..713459 + 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
LVJ74_RS03250 (LVJ74_03235) 715165..716454 - 1290 WP_003416640.1 ATP-binding protein -
LVJ74_RS03255 (LVJ74_03240) 716742..717035 - 294 WP_003416635.1 hypothetical protein -
LVJ74_RS03260 (LVJ74_03245) 717048..717259 - 212 Protein_642 (R)-hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 692482..714878 22396
- inside IScluster/Tn - - 708783..714878 6095


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T229087 WP_003899954.1 NZ_CP089779:c712339-711995 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T229087 NZ_CP124386:c35869-35720 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT229087 WP_003899955.1 NZ_CP089779:c711998-711669 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT229087 NZ_CP124386:35913-35974 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
AlphaFold DB G0THF6

References