Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 3562415..3563085 | Replicon | chromosome |
| Accession | NZ_CP089778 | ||
| Organism | Mycobacterium tuberculosis strain I0003758-5 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | O53332 |
| Locus tag | LVJ73_RS16860 | Protein ID | WP_003899954.1 |
| Coordinates | 3562415..3562759 (+) | Length | 115 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | G0THF6 |
| Locus tag | LVJ73_RS16865 | Protein ID | WP_003899955.1 |
| Coordinates | 3562756..3563085 (+) | Length | 110 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LVJ73_RS16825 (LVJ73_16830) | 3557488..3558348 | + | 861 | WP_003416628.1 | alpha/beta hydrolase | - |
| LVJ73_RS16830 (LVJ73_16835) | 3558323..3558838 | + | 516 | WP_003899950.1 | nitroreductase family deazaflavin-dependent oxidoreductase | - |
| LVJ73_RS16835 (LVJ73_16840) | 3558854..3559065 | + | 212 | Protein_3324 | (R)-hydratase | - |
| LVJ73_RS16840 (LVJ73_16845) | 3559078..3559371 | + | 294 | WP_003416635.1 | hypothetical protein | - |
| LVJ73_RS16845 (LVJ73_16850) | 3559659..3560948 | + | 1290 | WP_003416640.1 | ATP-binding protein | - |
| LVJ73_RS16850 (LVJ73_16855) | 3561295..3561729 | - | 435 | WP_003899952.1 | type II toxin-antitoxin system VapC family toxin | - |
| LVJ73_RS16855 (LVJ73_16860) | 3561732..3562184 | - | 453 | WP_003899953.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| LVJ73_RS16860 (LVJ73_16865) | 3562415..3562759 | + | 345 | WP_003899954.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LVJ73_RS16865 (LVJ73_16870) | 3562756..3563085 | + | 330 | WP_003899955.1 | XRE family transcriptional regulator | Antitoxin |
| LVJ73_RS16870 (LVJ73_16875) | 3563623..3563970 | + | 348 | WP_003899956.1 | DUF2384 domain-containing protein | - |
| LVJ73_RS16875 (LVJ73_16880) | 3563967..3564587 | + | 621 | WP_003899957.1 | RES family NAD+ phosphorylase | - |
| LVJ73_RS16880 (LVJ73_16885) | 3564747..3566012 | - | 1266 | WP_003899958.1 | hypothetical protein | - |
| LVJ73_RS16885 (LVJ73_16890) | 3566180..3566389 | + | 210 | WP_003416778.1 | hypothetical protein | - |
| LVJ73_RS16890 (LVJ73_16895) | 3566636..3567670 | - | 1035 | WP_003416786.1 | IS30 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 115 a.a. Molecular weight: 12692.50 Da Isoelectric Point: 5.6920
>T229078 WP_003899954.1 NZ_CP089778:3562415-3562759 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG
Download Length: 345 bp
>T229078 NZ_CP124385:c3385232-3384855 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA
Antitoxin
Download Length: 110 a.a. Molecular weight: 11802.46 Da Isoelectric Point: 7.4051
>AT229078 WP_003899955.1 NZ_CP089778:3562756-3563085 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA
Download Length: 330 bp
>AT229078 NZ_CP124385:c3385656-3385279 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U4FBK2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 6LTY | |
| PDB | 6LTZ | |
| AlphaFold DB | G0THF6 |