Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2209193..2210061 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag LVJ73_RS10295 Protein ID WP_010886136.1
Coordinates 2209193..2209570 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag LVJ73_RS10300 Protein ID WP_003409886.1
Coordinates 2209612..2210061 (+) Length 150 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS10245 (LVJ73_10260) 2204982..2205434 - 453 WP_003899095.1 lipoprotein -
LVJ73_RS10250 (LVJ73_10265) 2205498..2205899 + 402 WP_003409869.1 hypothetical protein -
LVJ73_RS10255 (LVJ73_10270) 2205892..2206074 - 183 WP_003409870.1 hypothetical protein -
LVJ73_RS10260 (LVJ73_10275) 2206188..2206538 - 351 WP_003899096.1 hypothetical protein -
LVJ73_RS10265 (LVJ73_10280) 2206549..2207451 - 903 WP_003899097.1 hypothetical protein -
LVJ73_RS10270 (LVJ73_10285) 2207472..2207663 - 192 WP_003409876.1 hypothetical protein -
LVJ73_RS10275 (LVJ73_10290) 2207664..2207960 - 297 WP_003409877.1 hypothetical protein -
LVJ73_RS10280 (LVJ73_10295) 2208200..2208415 + 216 WP_003409878.1 antitoxin -
LVJ73_RS10285 (LVJ73_10300) 2208412..2208723 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
LVJ73_RS10290 (LVJ73_10305) 2208697..2209218 - 522 WP_003904745.1 hypothetical protein -
LVJ73_RS10295 (LVJ73_10310) 2209193..2209570 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
LVJ73_RS10300 (LVJ73_10315) 2209612..2210061 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LVJ73_RS10305 (LVJ73_10320) 2210058..2210603 + 546 WP_003899098.1 SecB-like chaperone -
LVJ73_RS10310 (LVJ73_10325) 2210492..2211106 - 615 WP_003901296.1 hypothetical protein -
LVJ73_RS10315 (LVJ73_10330) 2211155..2211451 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
LVJ73_RS10320 (LVJ73_10335) 2211448..2211699 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
LVJ73_RS10325 (LVJ73_10340) 2211686..2212180 + 495 WP_003899099.1 hypothetical protein -
LVJ73_RS10330 (LVJ73_10345) 2212340..2212747 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
LVJ73_RS10335 (LVJ73_10350) 2212751..2213023 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ73_RS10340 (LVJ73_10355) 2213056..2214276 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-113)

Antitoxin

(44-91)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T229055 WP_010886136.1 NZ_CP089778:2209193-2209570 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T229055 NZ_CP124374:c4658653-4658546 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT229055 WP_003409886.1 NZ_CP089778:2209612-2210061 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT229055 NZ_CP124374:4658702-4658767 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU


Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References