Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3569880..3570550 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag LVJ72_RS16930 Protein ID WP_003899954.1
Coordinates 3569880..3570224 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag LVJ72_RS16935 Protein ID WP_003899955.1
Coordinates 3570221..3570550 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS16895 (LVJ72_16900) 3564953..3565813 + 861 WP_003416628.1 alpha/beta hydrolase -
LVJ72_RS16900 (LVJ72_16905) 3565788..3566303 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
LVJ72_RS16905 (LVJ72_16910) 3566319..3566530 + 212 Protein_3338 (R)-hydratase -
LVJ72_RS16910 (LVJ72_16915) 3566543..3566836 + 294 WP_003416635.1 hypothetical protein -
LVJ72_RS16915 (LVJ72_16920) 3567124..3568413 + 1290 WP_031664058.1 ATP-binding protein -
LVJ72_RS16920 (LVJ72_16925) 3568760..3569194 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
LVJ72_RS16925 (LVJ72_16930) 3569197..3569649 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ72_RS16930 (LVJ72_16935) 3569880..3570224 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ72_RS16935 (LVJ72_16940) 3570221..3570550 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
LVJ72_RS16945 (LVJ72_16950) 3572446..3572793 + 348 WP_003899956.1 DUF2384 domain-containing protein -
LVJ72_RS16950 (LVJ72_16955) 3572790..3573410 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
LVJ72_RS16955 (LVJ72_16960) 3573570..3574835 - 1266 Protein_3348 hypothetical protein -
LVJ72_RS16960 (LVJ72_16965) 3575003..3575212 + 210 WP_003416778.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T229018 WP_003899954.1 NZ_CP089777:3569880-3570224 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T229018 NZ_CP124372:c2427038-2426751 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT229018 WP_003899955.1 NZ_CP089777:3570221-3570550 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT229018 NZ_CP124372:c2426764-2426462 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References