Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2281723..2282642 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag LVJ72_RS10735 Protein ID WP_003900449.1
Coordinates 2282037..2282642 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag LVJ72_RS10730 Protein ID WP_003410124.1
Coordinates 2281723..2282028 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS10700 (LVJ72_10710) 2276734..2277990 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
LVJ72_RS10705 (LVJ72_10715) 2278344..2278919 + 576 WP_003410100.1 hypothetical protein -
LVJ72_RS10710 (LVJ72_10720) 2278916..2279956 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
LVJ72_RS10715 (LVJ72_10725) 2280198..2280917 + 720 WP_003410108.1 DUF433 domain-containing protein -
LVJ72_RS10720 (LVJ72_10730) 2280907..2281323 + 417 WP_003410114.1 hypothetical protein -
LVJ72_RS10725 (LVJ72_10735) 2281339..2281638 - 300 WP_003410120.1 hypothetical protein -
LVJ72_RS10730 (LVJ72_10740) 2281723..2282028 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
LVJ72_RS10735 (LVJ72_10745) 2282037..2282642 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ72_RS10740 (LVJ72_10750) 2282667..2283026 - 360 WP_003410131.1 hypothetical protein -
LVJ72_RS10745 (LVJ72_10755) 2283186..2283776 - 591 WP_057145698.1 hypothetical protein -
LVJ72_RS10750 (LVJ72_10760) 2283815..2284051 - 237 WP_003410133.1 hypothetical protein -
LVJ72_RS10755 (LVJ72_10765) 2284258..2285154 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T229000 WP_003900449.1 NZ_CP089777:c2282642-2282037 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T229000 NZ_CP124367:3771349-3771633 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT229000 WP_003410124.1 NZ_CP089777:c2282028-2281723 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT229000 NZ_CP124367:3771113-3771346 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References