Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3541251..3541921 Replicon chromosome
Accession NZ_CP089776
Organism Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag LVJ71_RS16785 Protein ID WP_003899954.1
Coordinates 3541251..3541595 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag LVJ71_RS16790 Protein ID WP_003899955.1
Coordinates 3541592..3541921 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ71_RS16750 (LVJ71_16750) 3536324..3537184 + 861 WP_003416628.1 alpha/beta hydrolase -
LVJ71_RS16755 (LVJ71_16755) 3537159..3537674 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
LVJ71_RS16760 (LVJ71_16760) 3537690..3537901 + 212 Protein_3309 (R)-hydratase -
LVJ71_RS16765 (LVJ71_16765) 3537914..3538207 + 294 WP_003416635.1 hypothetical protein -
LVJ71_RS16770 (LVJ71_16770) 3538495..3539784 + 1290 WP_003416640.1 ATP-binding protein -
LVJ71_RS16775 (LVJ71_16775) 3540131..3540565 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
LVJ71_RS16780 (LVJ71_16780) 3540568..3541020 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ71_RS16785 (LVJ71_16785) 3541251..3541595 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ71_RS16790 (LVJ71_16790) 3541592..3541921 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
LVJ71_RS16795 (LVJ71_16795) 3542459..3542806 + 348 WP_003899956.1 DUF2384 domain-containing protein -
LVJ71_RS16800 (LVJ71_16800) 3542803..3543423 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
LVJ71_RS16805 (LVJ71_16805) 3543583..3544848 - 1266 WP_003902423.1 hypothetical protein -
LVJ71_RS16810 (LVJ71_16810) 3545016..3545225 + 210 WP_003416778.1 hypothetical protein -
LVJ71_RS16815 (LVJ71_16815) 3545260..3545445 + 186 WP_003908177.1 hypothetical protein -
LVJ71_RS16820 (LVJ71_16820) 3545472..3546506 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T228958 WP_003899954.1 NZ_CP089776:3541251-3541595 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T228958 NZ_CP124359:188058-188165 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT228958 WP_003899955.1 NZ_CP089776:3541592-3541921 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT228958 NZ_CP124359:c188009-187944 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References