Detailed information of TA system TA022892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3150097..3150702
• Replicon: chromosome
• Accession: NC_010725
• Organism: Methylobacterium populi BJ001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B1ZEL9
• Locus tag: MPOP_RS14590
• Protein ID: WP_012454803.1
• Coordinates: 3150421..3150702 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MPOP_RS14585
• Protein ID: WP_012454802.1
• Coordinates: 3150097..3150390 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPOP_RS14555	3145718..3145933	-	216	WP_012454796.1	hypothetical protein	-
MPOP_RS14560	3146154..3146891	+	738	WP_012454797.1	cell wall hydrolase	-
MPOP_RS14565	3147026..3148030	+	1005	WP_012454798.1	lipoyl synthase	-
MPOP_RS14570	3148037..3148495	+	459	WP_012454799.1	type II toxin-antitoxin system RatA family toxin	-
MPOP_RS14575	3148496..3148921	-	426	WP_012454800.1	Lrp/AsnC family transcriptional regulator	-
MPOP_RS14580	3148963..3150084	+	1122	WP_205780417.1	ornithine cyclodeaminase	-
MPOP_RS14585	3150097..3150390	-	294	WP_012454802.1	HigA family addiction module antidote protein	Antitoxin
MPOP_RS14590	3150421..3150702	-	282	WP_012454803.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MPOP_RS14595	3150788..3151111	-	324	WP_012454804.1	hypothetical protein	-
MPOP_RS14600	3151108..3152895	-	1788	WP_041371459.1	ABC transporter ATP-binding protein/permease	-
MPOP_RS14605	3153526..3154020	+	495	WP_012454806.1	(2Fe-2S)-binding protein	-
MPOP_RS14610	3154017..3155066	+	1050	WP_012454807.1	xanthine dehydrogenase family protein subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11038.47 Da
• Isoelectric Point: 8.5134

>T22892 WP_012454803.1 NC_010725:c3150702-3150421 [Methylorubrum populi BJ001]
MIQSFANKRTRSLFEEGVCHRQWRAFERIARRKLDALDYAVVLDDLRSPPGNKLEALRGDRAGQYSIRINDQWRICFRWT
ESGPENVEIVDYH

Nucleotide

• Length: 282 bp

>T22892 NC_010725:c3150702-3150421 [Methylorubrum populi BJ001]
ATGATTCAAAGCTTCGCCAACAAGCGCACACGCTCTCTGTTCGAGGAAGGCGTCTGCCACCGTCAGTGGCGCGCCTTCGA
GCGAATCGCTCGGCGGAAGCTTGATGCTTTGGACTACGCCGTCGTGCTGGACGATCTGCGCAGCCCGCCGGGCAACAAGC
TTGAGGCTTTGCGCGGGGACAGAGCGGGGCAGTACAGCATTCGCATCAACGATCAGTGGCGGATTTGTTTCCGGTGGACT
GAGTCAGGCCCGGAGAATGTCGAGATCGTTGATTATCATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10925.39 Da
• Isoelectric Point: 9.8432

>AT22892 WP_012454802.1 NC_010725:c3150390-3150097 [Methylorubrum populi BJ001]
MIRVRTHPGEYLSEEYLKPLGLSARQLAVEIGVPANRITEIVRQRRGITADTALRLARYFGTTPQFWLNFQAGYDLSKAQ
AETDYSTVPQRRAADAA

Nucleotide

• Length: 294 bp

>AT22892 NC_010725:c3150390-3150097 [Methylorubrum populi BJ001]
ATGATTCGAGTTCGGACGCATCCTGGCGAGTACTTGTCGGAAGAATACCTCAAACCACTCGGTCTCAGTGCCCGCCAGCT
CGCGGTCGAGATCGGTGTTCCAGCCAACCGCATCACCGAGATCGTTCGGCAGCGACGCGGCATCACCGCAGATACGGCGC
TTCGTCTTGCACGCTACTTCGGGACAACCCCGCAGTTCTGGCTCAATTTTCAAGCCGGCTACGATCTCTCCAAGGCCCAG
GCGGAGACGGACTACTCAACTGTTCCACAGCGGCGAGCCGCCGACGCGGCGTGA

Structures

Toxin

• AlphaFold DB: B1ZEL9