Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2270078..2270997 Replicon chromosome
Accession NZ_CP089775
Organism Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag LVJ70_RS10645 Protein ID WP_003900449.1
Coordinates 2270392..2270997 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag LVJ70_RS10640 Protein ID WP_003410124.1
Coordinates 2270078..2270383 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ70_RS10610 (LVJ70_10620) 2265215..2266475 + 1261 Protein_2097 IS3-like element IS987 family transposase -
LVJ70_RS10615 (LVJ70_10625) 2266699..2267274 + 576 WP_015345103.1 hypothetical protein -
LVJ70_RS10620 (LVJ70_10630) 2267271..2268311 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
LVJ70_RS10625 (LVJ70_10635) 2268553..2269272 + 720 WP_003410108.1 DUF433 domain-containing protein -
LVJ70_RS10630 (LVJ70_10640) 2269262..2269678 + 417 WP_003410114.1 hypothetical protein -
LVJ70_RS10635 (LVJ70_10645) 2269694..2269993 - 300 WP_003410120.1 hypothetical protein -
LVJ70_RS10640 (LVJ70_10650) 2270078..2270383 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
LVJ70_RS10645 (LVJ70_10655) 2270392..2270997 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ70_RS10650 (LVJ70_10660) 2271022..2271381 - 360 WP_003410131.1 hypothetical protein -
LVJ70_RS10655 (LVJ70_10665) 2271541..2272131 - 591 WP_057145698.1 hypothetical protein -
LVJ70_RS10660 (LVJ70_10670) 2272170..2272406 - 237 WP_003410133.1 hypothetical protein -
LVJ70_RS10665 (LVJ70_10675) 2272613..2273509 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2261303..2273509 12206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T228879 WP_003900449.1 NZ_CP089775:c2270997-2270392 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T228879 NZ_CP124351:c5211972-5211865 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT228879 WP_003410124.1 NZ_CP089775:c2270383-2270078 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT228879 NZ_CP124351:5212020-5212086 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References