Detailed information of TA system TA022886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Gp49-HTH_37
• Location: 1099420..1099945
• Replicon: chromosome
• Accession: NC_010718
• Organism: Natranaerobius thermophilus JW/NM-WN-LF

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: NTHER_RS05270
• Protein ID: WP_202943886.1
• Coordinates: 1099679..1099945 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NTHER_RS05265
• Protein ID: WP_012447492.1
• Coordinates: 1099420..1099689 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NTHER_RS05235	1095353..1095859	-	507	WP_012447486.1	RNA polymerase sigma factor	-
NTHER_RS05240	1096369..1097220	+	852	WP_012447487.1	tyrosine-type recombinase/integrase	-
NTHER_RS05245	1097458..1097853	-	396	WP_012447488.1	HEPN domain-containing protein	-
NTHER_RS05250	1097825..1098160	-	336	WP_012447489.1	nucleotidyltransferase domain-containing protein	-
NTHER_RS05255	1098512..1098757	+	246	WP_041366924.1	hypothetical protein	-
NTHER_RS05260	1098760..1099146	+	387	WP_012447491.1	retropepsin-like domain-containing protein	-
NTHER_RS05265	1099420..1099689	-	270	WP_012447492.1	helix-turn-helix transcriptional regulator	Antitoxin
NTHER_RS05270	1099679..1099945	-	267	WP_202943886.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NTHER_RS05275	1100262..1101470	-	1209	WP_012447495.1	IS110 family transposase	-
NTHER_RS05280	1102010..1102258	+	249	WP_012447496.1	hypothetical protein	-
NTHER_RS05285	1102561..1102851	+	291	WP_012447497.1	nucleotidyltransferase domain-containing protein	-
NTHER_RS05290	1102844..1103176	+	333	WP_012447498.1	DUF86 domain-containing protein	-
NTHER_RS05295	1103571..1103828	+	258	WP_012447499.1	hypothetical protein	-
NTHER_RS05300	1104179..1104427	+	249	WP_012447501.1	hypothetical protein	-
NTHER_RS05305	1104424..1104804	+	381	WP_012447502.1	retropepsin-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (1-77)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10615.44 Da
• Isoelectric Point: 10.3352

>T22886 WP_202943886.1 NC_010718:c1099945-1099679 [Natranaerobius thermophilus JW/NM-WN-LF]
ILREIDLLEEFGLSLGMPHIRKIKNTELWELRIKHSSNIFRILFKDYNNTFVLLHGFQKKDNKTPKKDLNMALKRLKKYL
ERGDNYEP

Nucleotide

• Length: 267 bp

>T22886 NC_010718:c1099945-1099679 [Natranaerobius thermophilus JW/NM-WN-LF]
ATATTACGAGAAATTGATCTTCTTGAAGAATTTGGACTTTCTCTAGGGATGCCACATATTCGTAAAATTAAAAATACAGA
ACTTTGGGAATTAAGGATCAAACATAGTTCTAACATATTTCGAATACTTTTCAAAGATTATAATAATACCTTTGTTTTAC
TCCACGGTTTTCAAAAGAAAGATAACAAAACCCCTAAAAAAGATTTGAATATGGCTTTAAAAAGGTTAAAAAAGTATCTT
GAAAGAGGTGATAATTATGAACCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10519.21 Da
• Isoelectric Point: 10.0091

>AT22886 WP_012447492.1 NC_010718:c1099689-1099420 [Natranaerobius thermophilus JW/NM-WN-LF]
MNHKKVKEKLFNNPNVKKEYDNLEVLYDIKRQVIKLRKEKGLSQKELAEKIGTKQSAISRLENESYNPSIELLYKIAKAC
DRDLHIKFD

Nucleotide

• Length: 270 bp

>AT22886 NC_010718:c1099689-1099420 [Natranaerobius thermophilus JW/NM-WN-LF]
ATGAACCATAAGAAAGTTAAAGAAAAACTATTTAACAACCCTAATGTTAAAAAAGAATATGATAACTTAGAAGTCCTTTA
TGATATCAAACGACAAGTTATTAAGCTTCGCAAGGAAAAAGGTTTAAGTCAAAAAGAATTAGCTGAAAAAATAGGAACAA
AACAATCTGCAATCTCCCGATTAGAAAACGAAAGCTACAATCCAAGTATAGAGCTTTTATATAAAATAGCCAAAGCTTGC
GACAGAGATCTTCATATTAAATTTGATTAA