Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2265568..2266487 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag LVJ69_RS10650 Protein ID WP_003900449.1
Coordinates 2265882..2266487 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag LVJ69_RS10645 Protein ID WP_003410124.1
Coordinates 2265568..2265873 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS10615 (LVJ69_10625) 2260704..2261965 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
LVJ69_RS10620 (LVJ69_10630) 2262189..2262764 + 576 WP_015345103.1 hypothetical protein -
LVJ69_RS10625 (LVJ69_10635) 2262761..2263801 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
LVJ69_RS10630 (LVJ69_10640) 2264043..2264762 + 720 WP_003410108.1 DUF433 domain-containing protein -
LVJ69_RS10635 (LVJ69_10645) 2264752..2265168 + 417 WP_003410114.1 hypothetical protein -
LVJ69_RS10640 (LVJ69_10650) 2265184..2265483 - 300 WP_003410120.1 hypothetical protein -
LVJ69_RS10645 (LVJ69_10655) 2265568..2265873 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
LVJ69_RS10650 (LVJ69_10660) 2265882..2266487 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ69_RS10655 (LVJ69_10665) 2266512..2266871 - 360 WP_003410131.1 hypothetical protein -
LVJ69_RS10660 (LVJ69_10670) 2267031..2267621 - 591 WP_057145698.1 hypothetical protein -
LVJ69_RS10665 (LVJ69_10675) 2267660..2267896 - 237 WP_003410133.1 hypothetical protein -
LVJ69_RS10670 (LVJ69_10680) 2268103..2268999 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T228818 WP_003900449.1 NZ_CP089774:c2266487-2265882 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T228818 NZ_CP124347:2267107-2267209 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT228818 WP_003410124.1 NZ_CP089774:c2265873-2265568 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT228818 NZ_CP124347:c2267247-2266979 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References