Detailed information of TA system TA022881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 334587..335176
• Replicon: chromosome
• Accession: NC_010717
• Organism: Xanthomonas oryzae pv. oryzae PXO99A

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: PXO_RS01405
• Protein ID: WP_012443754.1
• Coordinates: 334587..334868 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PXO_RS01410
• Protein ID: WP_011260746.1
• Coordinates: 334886..335176 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PXO_RS01400	332240..334492	+	2253	WP_012443753.1	exodeoxyribonuclease V subunit alpha	-
PXO_RS01405	334587..334868	+	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PXO_RS01410	334886..335176	+	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
PXO_RS01415	336105..337499	+	1395	WP_027703652.1	nucleoside hydrolase	-
PXO_RS01420	337949..338281	-	333	WP_011260743.1	hypothetical protein	-
PXO_RS01430	338617..338841	+	225	WP_044751358.1	hypothetical protein	-
PXO_RS01435	338979..339413	+	435	WP_011260742.1	PAAR domain-containing protein	-
PXO_RS01440	339416..339892	+	477	WP_011260741.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T22881 WP_012443754.1 NC_010717:334587-334868 [Xanthomonas oryzae pv. oryzae PXO99A]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T22881 NC_010717:334587-334868 [Xanthomonas oryzae pv. oryzae PXO99A]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCGCATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT22881 WP_011260746.1 NC_010717:334886-335176 [Xanthomonas oryzae pv. oryzae PXO99A]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT22881 NC_010717:334886-335176 [Xanthomonas oryzae pv. oryzae PXO99A]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5