Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3553532..3554202 Replicon chromosome
Accession NZ_CP089773
Organism Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag LVJ68_RS16875 Protein ID WP_003899954.1
Coordinates 3553532..3553876 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag LVJ68_RS16880 Protein ID WP_003899955.1
Coordinates 3553873..3554202 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ68_RS16840 (LVJ68_16840) 3548605..3549465 + 861 WP_003416628.1 alpha/beta hydrolase -
LVJ68_RS16845 (LVJ68_16845) 3549440..3549955 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
LVJ68_RS16850 (LVJ68_16850) 3549971..3550182 + 212 Protein_3327 (R)-hydratase -
LVJ68_RS16855 (LVJ68_16855) 3550195..3550488 + 294 WP_003416635.1 hypothetical protein -
LVJ68_RS16860 (LVJ68_16860) 3550776..3552065 + 1290 WP_003416640.1 ATP-binding protein -
LVJ68_RS16865 (LVJ68_16865) 3552412..3552846 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
LVJ68_RS16870 (LVJ68_16870) 3552849..3553301 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ68_RS16875 (LVJ68_16875) 3553532..3553876 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ68_RS16880 (LVJ68_16880) 3553873..3554202 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
LVJ68_RS16885 (LVJ68_16885) 3554740..3555087 + 348 WP_003899956.1 DUF2384 domain-containing protein -
LVJ68_RS16890 (LVJ68_16890) 3555084..3555704 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
LVJ68_RS16895 (LVJ68_16895) 3555864..3557129 - 1266 WP_003902423.1 hypothetical protein -
LVJ68_RS16900 (LVJ68_16900) 3557297..3557506 + 210 WP_003416778.1 hypothetical protein -
LVJ68_RS16905 (LVJ68_16905) 3557753..3558787 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T228775 WP_003899954.1 NZ_CP089773:3553532-3553876 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T228775 NZ_CP124343:2964956-2965234 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT228775 WP_003899955.1 NZ_CP089773:3553873-3554202 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT228775 NZ_CP124343:2964763-2964954 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References