Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2264735..2265654 Replicon chromosome
Accession NZ_CP089773
Organism Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag LVJ68_RS10665 Protein ID WP_003900449.1
Coordinates 2265049..2265654 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag LVJ68_RS10660 Protein ID WP_003410124.1
Coordinates 2264735..2265040 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ68_RS10630 (LVJ68_10640) 2259746..2261002 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
LVJ68_RS10635 (LVJ68_10645) 2261356..2261931 + 576 WP_003410100.1 hypothetical protein -
LVJ68_RS10640 (LVJ68_10650) 2261928..2262968 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
LVJ68_RS10645 (LVJ68_10655) 2263210..2263929 + 720 WP_003410108.1 DUF433 domain-containing protein -
LVJ68_RS10650 (LVJ68_10660) 2263919..2264335 + 417 WP_003410114.1 hypothetical protein -
LVJ68_RS10655 (LVJ68_10665) 2264351..2264650 - 300 WP_003410120.1 hypothetical protein -
LVJ68_RS10660 (LVJ68_10670) 2264735..2265040 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
LVJ68_RS10665 (LVJ68_10675) 2265049..2265654 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ68_RS10670 (LVJ68_10680) 2265679..2266038 - 360 WP_003410131.1 hypothetical protein -
LVJ68_RS10675 (LVJ68_10685) 2266198..2266788 - 591 WP_057145698.1 hypothetical protein -
LVJ68_RS10680 (LVJ68_10690) 2266827..2267063 - 237 WP_003410133.1 hypothetical protein -
LVJ68_RS10685 (LVJ68_10695) 2267270..2268166 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T228757 WP_003900449.1 NZ_CP089773:c2265654-2265049 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T228757 NZ_CP124341:c4125816-4125529 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT228757 WP_003410124.1 NZ_CP089773:c2265040-2264735 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT228757 NZ_CP124341:c4125542-4125240 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References