Detailed information of TA system TA228757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2264735..2265654
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: L7N4R2
• Locus tag: LVJ68_RS10665
• Protein ID: WP_003900449.1
• Coordinates: 2265049..2265654 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: LVJ68_RS10660
• Protein ID: WP_003410124.1
• Coordinates: 2264735..2265040 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS10630 (LVJ68_10640)	2259746..2261002	-	1257	WP_003410095.1	HNH endonuclease signature motif containing protein	-
LVJ68_RS10635 (LVJ68_10645)	2261356..2261931	+	576	WP_003410100.1	hypothetical protein	-
LVJ68_RS10640 (LVJ68_10650)	2261928..2262968	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
LVJ68_RS10645 (LVJ68_10655)	2263210..2263929	+	720	WP_003410108.1	DUF433 domain-containing protein	-
LVJ68_RS10650 (LVJ68_10660)	2263919..2264335	+	417	WP_003410114.1	hypothetical protein	-
LVJ68_RS10655 (LVJ68_10665)	2264351..2264650	-	300	WP_003410120.1	hypothetical protein	-
LVJ68_RS10660 (LVJ68_10670)	2264735..2265040	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
LVJ68_RS10665 (LVJ68_10675)	2265049..2265654	-	606	WP_003900449.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ68_RS10670 (LVJ68_10680)	2265679..2266038	-	360	WP_003410131.1	hypothetical protein	-
LVJ68_RS10675 (LVJ68_10685)	2266198..2266788	-	591	WP_057145698.1	hypothetical protein	-
LVJ68_RS10680 (LVJ68_10690)	2266827..2267063	-	237	WP_003410133.1	hypothetical protein	-
LVJ68_RS10685 (LVJ68_10695)	2267270..2268166	-	897	WP_003410136.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22744.96 Da
• Isoelectric Point: 7.3073

>T228757 WP_003900449.1 NZ_CP089773:c2265654-2265049 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T228757 NZ_CP124341:c4125816-4125529 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT228757 WP_003410124.1 NZ_CP089773:c2265040-2264735 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT228757 NZ_CP124341:c4125542-4125240 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A7U4BV20

Antitoxin

• AlphaFold DB: A0A7U4BV30