Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976631..2977279 Replicon chromosome
Accession NZ_CP089772
Organism Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag LVJ67_RS14025 Protein ID WP_003899414.1
Coordinates 2976631..2976954 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag LVJ67_RS14030 Protein ID WP_003899415.1
Coordinates 2977034..2977279 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ67_RS13995 (LVJ67_13990) 2971954..2972952 + 999 WP_003900538.1 tyrosine-type recombinase/integrase -
LVJ67_RS14000 (LVJ67_13995) 2972966..2973430 + 465 WP_003900539.1 ParB N-terminal domain-containing protein -
LVJ67_RS14005 (LVJ67_14000) 2973418..2973669 + 252 WP_003908028.1 hypothetical protein -
LVJ67_RS14010 (LVJ67_14005) 2973840..2975279 - 1440 WP_003899411.1 phage major capsid protein -
LVJ67_RS14015 (LVJ67_14010) 2975287..2975820 - 534 WP_003899412.1 HK97 family phage prohead protease -
LVJ67_RS14020 (LVJ67_14015) 2975973..2976464 - 492 WP_003900541.1 phage terminase small subunit P27 family -
LVJ67_RS14025 (LVJ67_14020) 2976631..2976954 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
LVJ67_RS14030 (LVJ67_14025) 2977034..2977279 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
LVJ67_RS14035 (LVJ67_14030) 2977276..2978703 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
LVJ67_RS14040 (LVJ67_14035) 2978705..2979097 - 393 WP_003899417.1 DUF2742 domain-containing protein -
LVJ67_RS14045 (LVJ67_14040) 2979094..2979354 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
LVJ67_RS14050 (LVJ67_14045) 2979371..2979733 - 363 WP_003900543.1 hypothetical protein -
LVJ67_RS14055 (LVJ67_14050) 2979736..2980863 - 1128 WP_003899420.1 site-specific integrase -
LVJ67_RS14060 (LVJ67_14055) 2981008..2981235 - 228 WP_003899421.1 hypothetical protein -
LVJ67_RS14065 (LVJ67_14060) 2981232..2981621 - 390 WP_003899422.1 hypothetical protein -
LVJ67_RS14070 (LVJ67_14065) 2981527..2981799 + 273 WP_003900544.1 hypothetical protein -
LVJ67_RS14075 (LVJ67_14070) 2981898..2982131 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2971954..2980863 8909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-69)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T228707 WP_003899414.1 NZ_CP089772:c2976954-2976631 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T228707 NZ_CP124337:c3586209-3585907 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT228707 WP_003899415.1 NZ_CP089772:c2977279-2977034 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT228707 NZ_CP124337:c3585871-3585530 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References