Detailed information of TA system TA228697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2267864..2268783
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A654U351
• Locus tag: LVJ67_RS10640
• Protein ID: WP_003906719.1
• Coordinates: 2268178..2268783 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: LVJ67_RS10635
• Protein ID: WP_003410124.1
• Coordinates: 2267864..2268169 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS10605 (LVJ67_10610)	2262875..2264131	-	1257	WP_003410095.1	HNH endonuclease signature motif containing protein	-
LVJ67_RS10610 (LVJ67_10615)	2264485..2265060	+	576	WP_003410100.1	hypothetical protein	-
LVJ67_RS10615 (LVJ67_10620)	2265057..2266097	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
LVJ67_RS10620 (LVJ67_10625)	2266339..2267058	+	720	WP_003410108.1	DUF433 domain-containing protein	-
LVJ67_RS10625 (LVJ67_10630)	2267048..2267464	+	417	WP_003410114.1	hypothetical protein	-
LVJ67_RS10630 (LVJ67_10635)	2267480..2267779	-	300	WP_003410120.1	hypothetical protein	-
LVJ67_RS10635 (LVJ67_10640)	2267864..2268169	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
LVJ67_RS10640 (LVJ67_10645)	2268178..2268783	-	606	WP_003906719.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ67_RS10645 (LVJ67_10650)	2268808..2269167	-	360	WP_003410131.1	hypothetical protein	-
LVJ67_RS10650 (LVJ67_10655)	2269327..2269917	-	591	WP_057145698.1	hypothetical protein	-
LVJ67_RS10655 (LVJ67_10660)	2269956..2270192	-	237	WP_003410133.1	hypothetical protein	-
LVJ67_RS10660 (LVJ67_10665)	2270399..2271295	-	897	WP_003906720.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22716.91 Da
• Isoelectric Point: 7.3073

>T228697 WP_003906719.1 NZ_CP089772:c2268783-2268178 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T228697 NZ_CP124337:645572-645886 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT228697 WP_003410124.1 NZ_CP089772:c2268169-2267864 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT228697 NZ_CP124337:645883-646299 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A654U351

Antitoxin

• AlphaFold DB: A0A7U4BV30