Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2267864..2268783 Replicon chromosome
Accession NZ_CP089772
Organism Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)


Gene name HigB2 Uniprot ID A0A654U351
Locus tag LVJ67_RS10640 Protein ID WP_003906719.1
Coordinates 2268178..2268783 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag LVJ67_RS10635 Protein ID WP_003410124.1
Coordinates 2267864..2268169 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ67_RS10605 (LVJ67_10610) 2262875..2264131 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
LVJ67_RS10610 (LVJ67_10615) 2264485..2265060 + 576 WP_003410100.1 hypothetical protein -
LVJ67_RS10615 (LVJ67_10620) 2265057..2266097 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
LVJ67_RS10620 (LVJ67_10625) 2266339..2267058 + 720 WP_003410108.1 DUF433 domain-containing protein -
LVJ67_RS10625 (LVJ67_10630) 2267048..2267464 + 417 WP_003410114.1 hypothetical protein -
LVJ67_RS10630 (LVJ67_10635) 2267480..2267779 - 300 WP_003410120.1 hypothetical protein -
LVJ67_RS10635 (LVJ67_10640) 2267864..2268169 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
LVJ67_RS10640 (LVJ67_10645) 2268178..2268783 - 606 WP_003906719.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ67_RS10645 (LVJ67_10650) 2268808..2269167 - 360 WP_003410131.1 hypothetical protein -
LVJ67_RS10650 (LVJ67_10655) 2269327..2269917 - 591 WP_057145698.1 hypothetical protein -
LVJ67_RS10655 (LVJ67_10660) 2269956..2270192 - 237 WP_003410133.1 hypothetical protein -
LVJ67_RS10660 (LVJ67_10665) 2270399..2271295 - 897 WP_003906720.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22716.91 Da        Isoelectric Point: 7.3073

>T228697 WP_003906719.1 NZ_CP089772:c2268783-2268178 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T228697 NZ_CP124337:645572-645886 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT228697 WP_003410124.1 NZ_CP089772:c2268169-2267864 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT228697 NZ_CP124337:645883-646299 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A654U351

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References