228643

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 645543..646129 Replicon chromosome
Accession NZ_CP089771
Organism Klebsiella pneumoniae strain HKE12

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag LVO50_RS03240 Protein ID WP_002920800.1
Coordinates 645543..645911 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag LVO50_RS03245 Protein ID WP_004174006.1
Coordinates 645908..646129 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO50_RS03220 (641046) 641046..642116 - 1071 WP_004213396.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
LVO50_RS03225 (642118) 642118..642963 - 846 WP_004174009.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LVO50_RS03230 (642960) 642960..643847 - 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LVO50_RS03235 (643954) 643954..645270 - 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LVO50_RS03240 (645543) 645543..645911 - 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LVO50_RS03245 (645908) 645908..646129 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVO50_RS03250 (646293) 646293..647006 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LVO50_RS03255 (647008) 647008..647775 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LVO50_RS03260 (647772) 647772..649049 - 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
LVO50_RS03265 (649046) 649046..649972 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 640309..661392 21083


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T228643 WP_002920800.1 NZ_CP089771:c645911-645543 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T228643 NZ_CP124330:2040-2318 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGAATTGGTTATTCAGTCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT228643 WP_004174006.1 NZ_CP089771:c646129-645908 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT228643 NZ_CP124330:1771-2040 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CTGCAGGACGTCAGCAATTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATTTTCCGCTGCTGCATGGCGGGAT
GAGATGAGCAGGAAGATTGCCGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References