Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3479615..3480212 Replicon chromosome
Accession NZ_CP089768
Organism Klebsiella pneumoniae strain HKE9

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LVO52_RS16930 Protein ID WP_004142563.1
Coordinates 3479895..3480212 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LVO52_RS16925 Protein ID WP_004142561.1
Coordinates 3479615..3479902 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO52_RS16895 (3475695) 3475695..3475943 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LVO52_RS16900 (3475961) 3475961..3476302 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LVO52_RS16905 (3476333) 3476333..3477448 - 1116 WP_277693942.1 MBL fold metallo-hydrolase -
LVO52_RS16910 (3477628) 3477628..3478209 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
LVO52_RS16915 (3478209) 3478209..3478577 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LVO52_RS16920 (3478697) 3478697..3479350 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
LVO52_RS16925 (3479615) 3479615..3479902 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LVO52_RS16930 (3479895) 3479895..3480212 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVO52_RS16935 (3480397) 3480397..3481440 - 1044 WP_065810347.1 DUF2157 domain-containing protein -
LVO52_RS16940 (3482107) 3482107..3482973 - 867 WP_032105285.1 helix-turn-helix transcriptional regulator -
LVO52_RS16945 (3483082) 3483082..3484509 + 1428 WP_032411778.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T228632 WP_004142563.1 NZ_CP089768:c3480212-3479895 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T228632 NZ_CP124328:3414743-3414850 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT228632 WP_004142561.1 NZ_CP089768:c3479902-3479615 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT228632 NZ_CP124328:c3414694-3414629 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References