Detailed information of TA system TA228600

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 134613..134866
• Replicon: plasmid unnamed1
• Accession: NZ_CP089763
• Organism: Klebsiella pneumoniae strain HKC1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LVO51_RS28495
• Protein ID: WP_001312851.1
• Coordinates: 134717..134866 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	134613..134672 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVO51_RS28455 (130273)	130273..130338	-	66	Protein_177	helix-turn-helix domain-containing protein	-
LVO51_RS28460 (130391)	130391..131095	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LVO51_RS28465 (131120)	131120..131320	+	201	WP_072354025.1	hypothetical protein	-
LVO51_RS28470 (131340)	131340..132086	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LVO51_RS28475 (132141)	132141..132701	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LVO51_RS28480 (132833)	132833..133033	+	201	WP_015059022.1	hypothetical protein	-
LVO51_RS28485 (133419)	133419..134018	+	600	WP_032083981.1	PIN domain-containing protein	-
LVO51_RS28490 (134080)	134080..134412	+	333	WP_152916585.1	hypothetical protein	-
- (134613)	134613..134672	-	60	NuclAT_1	-	Antitoxin
- (134613)	134613..134672	-	60	NuclAT_1	-	Antitoxin
- (134613)	134613..134672	-	60	NuclAT_1	-	Antitoxin
- (134613)	134613..134672	-	60	NuclAT_1	-	Antitoxin
LVO51_RS28495 (134717)	134717..134866	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LVO51_RS28500 (135150)	135150..135398	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaKPC-2	-	1..135709	135709

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T228600 WP_001312851.1 NZ_CP089763:134717-134866 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T228600 NZ_CP124326:976007-976414 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT228600 NZ_CP089763:c134672-134613 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin