Detailed information of TA system TA228596

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35049..35318
• Replicon: plasmid unnamed1
• Accession: NZ_CP089763
• Organism: Klebsiella pneumoniae strain HKC1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LVO51_RS27800
• Protein ID: WP_001372321.1
• Coordinates: 35193..35318 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	35049..35114 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVO51_RS27770	30759..31286	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LVO51_RS27775	31344..31577	+	234	WP_000006003.1	DUF905 family protein	-
LVO51_RS27780	31638..33661	+	2024	Protein_42	ParB/RepB/Spo0J family partition protein	-
LVO51_RS27785	33730..34164	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LVO51_RS27790	34161..34880	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	34892..35116	+	225	NuclAT_0	-	-
-	34892..35116	+	225	NuclAT_0	-	-
-	34892..35116	+	225	NuclAT_0	-	-
-	34892..35116	+	225	NuclAT_0	-	-
-	35049..35114	-	66	-	-	Antitoxin
LVO51_RS27795	35102..35251	+	150	Protein_45	plasmid maintenance protein Mok	-
LVO51_RS27800	35193..35318	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LVO51_RS27805	35637..35933	-	297	Protein_47	hypothetical protein	-
LVO51_RS27810	36374..37078	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LVO51_RS27815	37144..37428	+	285	Protein_49	PAS domain-containing protein	-
LVO51_RS27820	37492..38196	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LVO51_RS27825	38287..38904	+	618	Protein_51	replication protein	-
LVO51_RS27830	39358..39636	+	279	WP_013213990.1	hypothetical protein	-
LVO51_RS27835	39747..40172	+	426	WP_013213989.1	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaKPC-2	-	1..135709	135709

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T228596 WP_001372321.1 NZ_CP089763:35193-35318 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T228596 NZ_CP124326:291052-291579 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT228596 NZ_CP089763:c35114-35049 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin