Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 5042878..5043464 Replicon chromosome
Accession NZ_CP089762
Organism Klebsiella pneumoniae strain HKC1

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag LVO51_RS24865 Protein ID WP_002920800.1
Coordinates 5043096..5043464 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag LVO51_RS24860 Protein ID WP_002920802.1
Coordinates 5042878..5043099 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO51_RS24840 (LVO51_24805) 5039035..5039961 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LVO51_RS24845 (LVO51_24810) 5039958..5041235 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
LVO51_RS24850 (LVO51_24815) 5041232..5041999 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LVO51_RS24855 (LVO51_24820) 5042001..5042714 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LVO51_RS24860 (LVO51_24825) 5042878..5043099 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVO51_RS24865 (LVO51_24830) 5043096..5043464 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LVO51_RS24870 (LVO51_24835) 5043737..5045053 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LVO51_RS24875 (LVO51_24840) 5045160..5046047 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LVO51_RS24880 (LVO51_24845) 5046044..5046889 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LVO51_RS24885 (LVO51_24850) 5046891..5047961 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 5039958..5048698 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T228593 WP_002920800.1 NZ_CP089762:5043096-5043464 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T228593 NZ_CP124325:c142290-141985 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT228593 WP_002920802.1 NZ_CP089762:5042878-5043099 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT228593 NZ_CP124325:c142510-142292 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References