Detailed information of TA system TA228577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5328039..5328634
• Replicon: chromosome
• Accession: NZ_CP089745
• Organism: Pseudomonas aeruginosa strain Pa608

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: LRP95_RS24755
• Protein ID: WP_003117425.1
• Coordinates: 5328356..5328634 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRP95_RS24750
• Protein ID: WP_003113527.1
• Coordinates: 5328039..5328344 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRP95_RS24715	5323100..5323534	-	435	WP_031635504.1	hypothetical protein	-
LRP95_RS24720	5324073..5324717	-	645	WP_021264266.1	DNA cytosine methyltransferase	-
LRP95_RS24725	5324725..5325009	-	285	WP_232683422.1	DUF5447 family protein	-
LRP95_RS24730	5325036..5325308	-	273	WP_004352675.1	hypothetical protein	-
LRP95_RS24735	5325418..5325684	+	267	WP_023088595.1	hypothetical protein	-
LRP95_RS24740	5325740..5326075	+	336	WP_232683423.1	hypothetical protein	-
LRP95_RS24745	5326209..5327483	-	1275	WP_232683424.1	RelA/SpoT domain-containing protein	-
LRP95_RS24750	5328039..5328344	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
LRP95_RS24755	5328356..5328634	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRP95_RS24760	5328687..5328815	-	129	Protein_4897	integrase	-
LRP95_RS24765	5328963..5331191	+	2229	WP_003113525.1	TonB-dependent receptor	-
LRP95_RS24770	5331261..5331908	-	648	WP_003095021.1	carbonate dehydratase	-
LRP95_RS24775	5331970..5333208	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T228577 WP_003117425.1 NZ_CP089745:c5328634-5328356 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T228577 NZ_CP124324:977677-978084 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT228577 WP_003113527.1 NZ_CP089745:c5328344-5328039 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT228577 NZ_CP124324:977430-977696 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5