Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3592691..3593314 Replicon chromosome
Accession NZ_CP089742
Organism Melaminivora jejuensis strain KACC 19629

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag LVC68_RS16540 Protein ID WP_209423446.1
Coordinates 3592691..3592972 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag LVC68_RS16545 Protein ID WP_209423445.1
Coordinates 3593003..3593314 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVC68_RS16520 (LVC68_16520) 3588430..3589005 - 576 WP_209423449.1 type I-F CRISPR-associated endoribonuclease Cas6/Csy4 -
LVC68_RS16525 (LVC68_16525) 3589020..3590045 - 1026 WP_232653850.1 type I-F CRISPR-associated protein Csy3 -
LVC68_RS16530 (LVC68_16530) 3590112..3591182 - 1071 WP_209423447.1 type I-F CRISPR-associated protein Csy2 -
LVC68_RS16535 (LVC68_16535) 3591179..3592540 - 1362 WP_232653852.1 type I-F CRISPR-associated protein Csy1 -
LVC68_RS16540 (LVC68_16540) 3592691..3592972 + 282 WP_209423446.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVC68_RS16545 (LVC68_16545) 3593003..3593314 + 312 WP_209423445.1 HigA family addiction module antitoxin Antitoxin
LVC68_RS16550 (LVC68_16550) 3593330..3596845 - 3516 WP_209423444.1 type I-F CRISPR-associated helicase Cas3f -
LVC68_RS16555 (LVC68_16555) 3596842..3597834 - 993 WP_209424145.1 type I-F CRISPR-associated endonuclease Cas1f -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(19-67)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10588.03 Da        Isoelectric Point: 8.5517

>T228569 WP_209423446.1 NZ_CP089742:3592691-3592972 [Melaminivora jejuensis]
MAVVSFQCPDTQRLFTSGKTRRFANIQAVAERKLVQRDAAATLDFLRSPPGNRLEALTGDRQGQHSIRINAQWRVCFVWM
DEGPAQVEIVDYH

Download         Length: 282 bp

>T228569 NZ_CP124322:c4549030-4548857 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11525.42 Da        Isoelectric Point: 9.9120

>AT228569 WP_209423445.1 NZ_CP089742:3593003-3593314 [Melaminivora jejuensis]
MFKNGMRPVHPGEVLREDYLKPLALSANALARQLRVPASRINDIVLGRRGITADTALRLCRFFGGDPQSWLNLQTAYDLR
MAQMDEALQQAVLAVQPMQLHPA

Download         Length: 312 bp

>AT228569 NZ_CP124322:c4549354-4549058 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References