Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2267971..2268890 Replicon chromosome
Accession NZ_CP089611
Organism Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag LVJ90_RS10635 Protein ID WP_003900449.1
Coordinates 2268285..2268890 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag LVJ90_RS10630 Protein ID WP_003410124.1
Coordinates 2267971..2268276 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ90_RS10600 (LVJ90_10610) 2262982..2264238 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
LVJ90_RS10605 (LVJ90_10615) 2264592..2265167 + 576 WP_015345103.1 hypothetical protein -
LVJ90_RS10610 (LVJ90_10620) 2265164..2266204 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
LVJ90_RS10615 (LVJ90_10625) 2266446..2267165 + 720 WP_003410108.1 DUF433 domain-containing protein -
LVJ90_RS10620 (LVJ90_10630) 2267155..2267571 + 417 WP_003410114.1 hypothetical protein -
LVJ90_RS10625 (LVJ90_10635) 2267587..2267886 - 300 WP_003410120.1 hypothetical protein -
LVJ90_RS10630 (LVJ90_10640) 2267971..2268276 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
LVJ90_RS10635 (LVJ90_10645) 2268285..2268890 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ90_RS10640 (LVJ90_10650) 2268915..2269274 - 360 WP_003410131.1 hypothetical protein -
LVJ90_RS10645 (LVJ90_10655) 2269434..2270024 - 591 WP_057145698.1 hypothetical protein -
LVJ90_RS10650 (LVJ90_10660) 2270063..2270299 - 237 WP_003410133.1 hypothetical protein -
LVJ90_RS10655 (LVJ90_10665) 2270506..2271402 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T228539 WP_003900449.1 NZ_CP089611:c2268890-2268285 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T228539 NZ_CP124320:c2948865-2948763 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT228539 WP_003410124.1 NZ_CP089611:c2268276-2267971 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT228539 NZ_CP124320:2948725-2948993 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References