Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2274269..2275188 Replicon chromosome
Accession NZ_CP089610
Organism Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag LVJ91_RS10665 Protein ID WP_003900449.1
Coordinates 2274583..2275188 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag LVJ91_RS10660 Protein ID WP_003410124.1
Coordinates 2274269..2274574 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ91_RS10630 (LVJ91_10645) 2269649..2270536 - 888 Protein_2101 13E12 repeat family protein -
LVJ91_RS10635 (LVJ91_10650) 2270890..2271465 + 576 WP_003410100.1 hypothetical protein -
LVJ91_RS10640 (LVJ91_10655) 2271462..2272502 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
LVJ91_RS10645 (LVJ91_10660) 2272744..2273463 + 720 WP_003410108.1 DUF433 domain-containing protein -
LVJ91_RS10650 (LVJ91_10665) 2273453..2273869 + 417 WP_003410114.1 hypothetical protein -
LVJ91_RS10655 (LVJ91_10670) 2273885..2274184 - 300 WP_003410120.1 hypothetical protein -
LVJ91_RS10660 (LVJ91_10675) 2274269..2274574 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
LVJ91_RS10665 (LVJ91_10680) 2274583..2275188 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ91_RS10670 (LVJ91_10685) 2275213..2275572 - 360 WP_003410131.1 hypothetical protein -
LVJ91_RS10675 (LVJ91_10690) 2275732..2276364 - 633 WP_003913310.1 hypothetical protein -
LVJ91_RS10680 (LVJ91_10695) 2276361..2276597 - 237 WP_003410133.1 hypothetical protein -
LVJ91_RS10685 (LVJ91_10700) 2276804..2277700 - 897 WP_003906720.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T228478 WP_003900449.1 NZ_CP089610:c2275188-2274583 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T228478 NZ_CP123969:c250324-249989 [Streptococcus agalactiae]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCCCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAATGACATAGAAAAACTTGAAGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAGAAATCAGCAATTATCACAGTACTAGAGGTTACACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAAGAGGAAGAGAATAGCGTTGTCATTGATTACTTGCTTCCTACTCGAAGCGACTATAT
GAAATTGTTTAAATAG

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT228478 WP_003410124.1 NZ_CP089610:c2274574-2274269 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT228478 NZ_CP123969:c250601-250314 [Streptococcus agalactiae]
ATGGTTACAGTAGAAAAAAATAGAGCTGTCACATTCCAAGCTAACAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCCGAAATCAGCAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References