Detailed information of TA system TA228437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 22688..23345
• Replicon: plasmid pVFN3-blaOXA-193K
• Accession: NZ_CP089604
• Organism: Vibrio furnissii strain VFN3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: LUM42_RS16080
• Protein ID: WP_000270043.1
• Coordinates: 22995..23345 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LUM42_RS16075
• Protein ID: WP_000124640.1
• Coordinates: 22688..22990 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUM42_RS16030 (LUM42_16030)	18313..18741	+	429	WP_000591074.1	hypothetical protein	-
LUM42_RS16035 (LUM42_16035)	18798..19157	+	360	WP_000422768.1	hypothetical protein	-
LUM42_RS16040 (LUM42_16040)	19157..19603	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
LUM42_RS16045 (LUM42_16045)	19600..20118	+	519	WP_000210756.1	nitrite reductase	-
LUM42_RS16050 (LUM42_16050)	20118..20348	+	231	WP_000972663.1	hypothetical protein	-
LUM42_RS16055 (LUM42_16055)	20335..21192	+	858	WP_001167032.1	hypothetical protein	-
LUM42_RS16060 (LUM42_16060)	21423..21950	+	528	WP_001236377.1	thermonuclease family protein	-
LUM42_RS16065 (LUM42_16065)	22008..22280	+	273	WP_001043047.1	HU family DNA-binding protein	-
LUM42_RS16070 (LUM42_16070)	22368..22661	+	294	WP_001239997.1	chromosome segregation protein ParM	-
LUM42_RS16075 (LUM42_16075)	22688..22990	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
LUM42_RS16080 (LUM42_16080)	22995..23345	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LUM42_RS16085 (LUM42_16085)	23508..24056	+	549	WP_001061574.1	transcriptional regulator	-
LUM42_RS16090 (LUM42_16090)	24397..24591	+	195	WP_000343597.1	hypothetical protein	-
LUM42_RS16095 (LUM42_16095)	24602..24973	+	372	WP_000516916.1	hypothetical protein	-
LUM42_RS16100 (LUM42_16100)	24966..25436	+	471	WP_001281821.1	hypothetical protein	-
LUM42_RS16105 (LUM42_16105)	25451..25786	-	336	WP_000683476.1	hypothetical protein	-
LUM42_RS16110 (LUM42_16110)	25883..26371	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
LUM42_RS16115 (LUM42_16115)	26374..26871	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / tet(D) / floR / sul2 / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / mph(A)	-	1..193896	193896

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T228437 WP_000270043.1 NZ_CP089604:c23345-22995 [Vibrio furnissii]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T228437 NZ_CP123953:2924043-2924798 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAGCAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGCCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT228437 WP_000124640.1 NZ_CP089604:c22990-22688 [Vibrio furnissii]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT228437 NZ_CP123953:2923540-2923908 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGGGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9