Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 58746..59473 | Replicon | plasmid pKP29499-1 |
| Accession | NZ_CP089599 | ||
| Organism | Klebsiella pneumoniae strain KP29499 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | LVE73_RS26845 | Protein ID | WP_011251285.1 |
| Coordinates | 59162..59473 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | LVE73_RS26840 | Protein ID | WP_011251286.1 |
| Coordinates | 58746..59165 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LVE73_RS26810 (LVE73_26810) | 53914..54384 | - | 471 | WP_048333570.1 | hypothetical protein | - |
| LVE73_RS26815 (LVE73_26815) | 54697..55332 | - | 636 | WP_223171879.1 | hypothetical protein | - |
| LVE73_RS26820 (LVE73_26820) | 55751..56371 | + | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| LVE73_RS26825 (LVE73_26825) | 56392..57180 | + | 789 | WP_040217257.1 | hypothetical protein | - |
| LVE73_RS26830 (LVE73_26830) | 57194..57559 | + | 366 | WP_048333448.1 | hypothetical protein | - |
| LVE73_RS26835 (LVE73_26835) | 57631..58599 | - | 969 | WP_074428168.1 | IS5 family transposase | - |
| LVE73_RS26840 (LVE73_26840) | 58746..59165 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| LVE73_RS26845 (LVE73_26845) | 59162..59473 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| LVE73_RS26850 (LVE73_26850) | 59678..60115 | - | 438 | Protein_59 | DDE-type integrase/transposase/recombinase | - |
| LVE73_RS26855 (LVE73_26855) | 60250..60947 | + | 698 | WP_223175001.1 | IS1-like element IS1A family transposase | - |
| LVE73_RS26860 (LVE73_26860) | 60949..61398 | + | 450 | WP_011251283.1 | thioredoxin fold domain-containing protein | - |
| LVE73_RS26865 (LVE73_26865) | 61415..61726 | + | 312 | WP_011251282.1 | hypothetical protein | - |
| LVE73_RS26870 (LVE73_26870) | 61740..62081 | + | 342 | WP_011251281.1 | hypothetical protein | - |
| LVE73_RS26875 (LVE73_26875) | 62141..63097 | + | 957 | WP_011251280.1 | DsbA family protein | - |
| LVE73_RS26880 (LVE73_26880) | 63214..63789 | + | 576 | WP_074424927.1 | hypothetical protein | - |
| LVE73_RS26885 (LVE73_26885) | 63897..64412 | + | 516 | WP_041937820.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..229581 | 229581 | |
| - | inside | IScluster/Tn | - | - | 47298..60947 | 13649 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T228428 WP_011251285.1 NZ_CP089599:c59473-59162 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T228428 NZ_CP123951:c3592123-3591947 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT228428 WP_011251286.1 NZ_CP089599:c59165-58746 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT228428 NZ_CP123951:c3591901-3591485 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|